Transmission electron microscopy analysis of T. cruzi epimastigotes treated with NQ8. (A-D) Treatment with 0.8 μM NQ8 led to swelling in the mitochondrion (*) and the appearance of membranous structures inside the organelle (black arrows). The formation of atypical cytosolic membranous structures was also observed (white arrowheads) near to the washed out aspect of cytosol (black star). Blebs containing electron-dense material (thick black arrows) were found close to the flagellar pocket. Bars = 500 nm (A-C) and 200 nm (D).