Figure 6
The infectious properties of standard DENV2 and imDENV2 determined by qRT-PCR and plaque forming assay. The viral RNA copies determined by qRT-PCR (A) and the plaque morphology and infectious titer determined by plaque forming assay (B and C) of DENV2 produced in C6/36 and LoVo cells at each time point. (D) The ratio of viral RNA copies (copies/ml) to infectious titer (PFU) for the distinct virus preparations. The specific infectivity of imDENV2 was significant lower than that of DENV2 generated in C6/36 cells. Data are expressed as means of at least three independent experiments. The error bars represent standard deviations (SD). If there is no error bar, it is not that no variations among three independent experiments but that the variations are too small to show in the figure. * P < 0.05 vs C6/36.