Characterization and in vitro properties of oral lactobacilli in breastfed infants

BMC Microbiology

Table 3 L. gasseri adhesion to saliva coated hydroxyapatite and aggregation in saliva

	Parotid saliva		Submandibular/sublingual saliva
L. gasseri	Adhesion¹	Aggregation²	Adhesion¹	Aggregation²
Isolate B16	++	++	+++	+++
Isolate B1	+	+	++	++
Isolate L10	+	++	++	+++
Isolate A241	+	+	++	++
Isolate A274	+	++	++	+++
Type strain 31451^T	++	++	+++	+++

¹ 62.5×10⁶ bacterial cells were added into each test well. + binding of <15% of added bacterial cells, ++ ≥15 to <20%, and +++ ≥20%.
² – =aggregation score 0 (no visible aggregates), + aggregation score 1 (small uniform aggregates), ++ aggregation score 2 (more aggregates of slightly larger size than 1), +++ aggregation score 3 (more and slightly larger aggregates than 2) [30]. Adhesion buffer was used a negative control (score 0) and S. mutans strain Ingbritt as positive control (score +++) [18].

ISSN: 1471-2180