Figure 6
LP5 affects the supercoiling and decatenation activity of S . aureus DNA. (A) The supercoiling reaction mixtures containing relaxed DNA and S. aureus gyrase (Gyr) (Lane 2–8). Lane 1 served as a negative control containing only relaxed DNA. Lane 3 served as a positive control containing ciprofloxacin (Cip). Lane 4–8 containing increasing concentration of LP5 (66.4 μg/ml to 331.8 μg/ml). (B) The decatenation reaction mixtures containing kinetoplast DNA and S. aureus Topo IV (Lane 2–8). Lane 1 served as a negative control containing only relaxed DNA. Lane 3 served as a positive control containing ciprofloxacin (Cip). Lane 4–8 containing increasing concentration of LP5 (66.4 μg/ml to 331.8 μg/ml).