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Figure 1 | BMC Microbiology

Figure 1

From: Feasibility study demonstrating that enzymatic template generation and amplification can be employed as a novel method for molecular antimicrobial susceptibility testing

Figure 1

Experimental design of the study. On day one, the macrobroth AST is assembled. At the indicated time points, an aliquot is removed from each broth and diluted ten-fold. A portion of the diluted sample is subjected to bead milling for bacterial lysis, and incubated for ETGA substrate conversion. Once processed, the samples are stored at -20°C prior to analysis. On day two, the MIC of the AST is determined by visual turbidity. A final time point sample from each culture is taken and processed as day one, except the samples from any turbid cultures are diluted 1:10,000 fold. Once all samples are processed, the sample set is analyzed through the qPCR readout portion of the assay. These samples are also analyzed using the appropriate gene-specific qPCR assay as a comparison. The MIC as determined by the molecular AST analyses were compared to the MIC as determined from the predicate macrobroth analysis to determine the agreement between these methods.

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BMC Microbiology

ISSN: 1471-2180

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