Variability of antibiotic susceptibility and toxin production of Staphylococcus aureus strains isolated from skin, soft tissue, and bone related infections

Sina, Haziz; Ahoyo, Théodora A; Moussaoui, Wardi; Keller, Daniel; Bankolé, Honoré S; Barogui, Yves; Stienstra, Ymkje; Kotchoni, Simeon O; Prévost, Gilles; Baba-Moussa, Lamine

doi:10.1186/1471-2180-13-188

Table 1 Primers sequences used in this study for the detection of genes encoding toxins

From: Variability of antibiotic susceptibility and toxin production of Staphylococcus aureus strains isolated from skin, soft tissue, and bone related infections

Primers	Sequences	Polarity	Expected fragment (base pair)	Accession N°
*eta*	5′-GCAGGTGTTGATTTAGCATT-3′	Sens	93	A8658-043
*eta*	5′-AGATGTCCCTATTTTTGCTG-3′	Antisens	93	A8658-044
*etb*	5′-ACCCCTGTTCCCTTATCATC-3′	Sens	226	A8659-036
*etb*	5′-GTTTTTGGCTGCTTCTCTTG-3′	Antisens	226	A8659-037
*lpv*	5′-AAAATGCCACTGTTATCCAGAGGTA-3′	Sens	433	A8658-029
*lpv*	5′-TTTGCAGCGTTTTGTTTTCG-3′	Antisens	433	A8658-030
*sea*	5′-ATGGTTATCAATGTGCGGGTGIIIIICCAAACAAAAC-3′	Sens	520	A8658-031
*sea*	5′-TGAATACTGTCCTTGAGCACCAIIIIIATCGTAATTAAC-3′	Antisens	520	A8658-032
*seb*	5′-TGGTATGACATGATGCCTGCACIIIIIGATAAATTTGAC-3′	Sens	163	A8658-033
*seb*	5′-AGGTACTCTATAAGTGCCTGCCTIIIIIACTAACTCTT-3′	Antisens	163	A8658-034
*sec*	5′-GATGAAGTAGTTGATGTGTATGGATCIIIIIACTATGTAAAC-3′	Sens	283	A8658-035
*sec*	5′-AGATTGGTCAAACTTATCGCCTGGIIIIIGCATCATATC-3′	Antisens	283	A8658-036
*sed*	5′-CTGAATTAAGTAGTACCGCGCTIIIIIATATGAAAC-3′	Sens	384	A8658-037
*sed*	5′-TCCTTTTGCAAATAGCGCCTTGIIIIIGCATCTAATTC-3′	Antisens	384	A8658-038
*see*	5′-CTTACCGCCAAAGCTGTCG-3′	Sens	159	A8658-039
*see*	5′-GTCCACTTGTAAATGGTAGCGAGAA-3′	Antisens	159	A8658-040
*seg*	5′-AATTATGTGAATGCTCAACCCGAT-3′	Sens	408	HA01804615
*seg*	5′-CTTTAGTGAGCCAGTGTCTTGCTTTG-3′	Antisens		HA01804616
*seh*	5′- CATCTACCCAAACATTAGCACC-3′	Sens	222	HA01804617
*seh*	5′-AGAAATCAAGGTGATAGTGGCAA-3′	Antisens	222	HA01804618
*sei*	5′-AGGCGTCACAGATAAAAACCTACCIIIIICAAATCAACTC-3′	Sens	454	HA01804619
*sei*	5′-ACAAGGACCATTATAATCAATGCCIIIIITATCCAGTTTC-3′	Antisens	454	HA01804620
*tsst-1*	5′-ACCCCTGCCTTTCCATCATC-3′	Sens	209	A8658-041
*tsst-1*	5′-TTTTCAGTATTTGTAACGCC-3′	Antisens	209	A8658-042

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ISSN: 1471-2180

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