Transfection with EB1.84-GFP disrupts inclusion fusion. HeLa cells were transfected with EB1.84-GFP or mock transfected. They were then infected with C. trachomatis. Twenty-four hours postinfection, cells were fixed and stained with human sera and inclusions per infected cell were enumerated. The distribution in the number of inclusions per infected cell is shown for the EB1.84-GFP transfected and mock transfected cells in A and B, respectively. Mock transfected cells were also stained with anti-g-tubulin antibodies (green). Representative transfected and mock transfected cells shown in C and D, respectively.