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Table 3 Inhibition of Mpn and human HPRT by purine analogs *

From: Inhibition of Mycoplasma pneumoniae growth by FDA-approved anticancer and antiviral nucleoside and nucleobase analogs

Substrate Hypoxanthine Guanine
Analogs IC50(μM) IC50(μM)
  Mpn Human P value Mpn Human P value
6-thioguanine 3.5 ± 0.5 20.5 ± 6.5 0.0107 3.16 ± 0.2 39.8 ± 4 <0.0001
6-mercaptopurine 89.7 ± 14.5 22.5 ± 3.6 0.0015 281.8 ± 21 25.1 ± 3 <0.0001
Theophylline > 4000 1585 ± 134   nd nd  
Caffeine > 4000 2511 ± 156   nd nd  
  1. *Assays were performed with 10 μM tritium labelled hypoxanthine or guanine as substrates and various concentrations of the inhibitors. Data were mean ± standard deviation (SD) from at least three independent determinations. P < 0.05 is considered as significant.
  2. nd = not determined.