Figure 3
Substrate saturation curves of TFT with human TK2 (A), human TK1 (B), and Ureaplasma TK (C). Kinetic assays with TFT were performed by using [γ-32P]-ATP as the labelled substrate at fixed concentration and variable concentrations of TFT. The reaction products were separated by thin layer chromatography, and quantified as described in the experimental procedures. Data are from three independent measurements and are presented as mean ± SD.