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Table 2 Kinetic parameters of the wild-type and stabilized MetA enzymes

From: Stabilized homoserine o-succinyltransferases (MetA) or L-methionine partially recovers the growth defect in Escherichia coli lacking ATP-dependent proteases or the DnaK chaperone

Enzyme

kcat(s-1)

Succinyl-CoA

L-homoserine

  

Km(mM)

kcat/KM(M-1 s-1)

Km(mM)

kcat/KM(M-1 s-1)

MetA, wt

36.72 ± 0.9

0.37 ± 0.05

9.9*104

1.25 ± 0.3

2.93*104

I124L

38.59 ± 0.5

0.38 ± 0.06

1.02*105

0.83 ± 0.15

4.65*104

I229Y

39.28 ± 0.5

0.36 ± 0.06

1.09*105

1.42 ± 0.1

2.76*104

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BMC Microbiology

ISSN: 1471-2180

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