Stabilized homoserine o-succinyltransferases (MetA) or L-methionine partially recovers the growth defect in Escherichia coli lacking ATP-dependent proteases or the DnaK chaperone

Table 1 Differential scanning calorimetric data for the wild- type and mutant MetA enzymes

Enzyme	T_m(°C)	∆H^*	∆H_v^*	∆H/∆H_v
MetA, wt	47.01 ± 0.26	5.93 x 10⁴	1.18 x 10⁵	0.5
I124L	48.65 ± 0.06	6.51 x 10⁴	1.86 x 10⁵	0.35
I229Y	50.68 ± 0.06	8.99 x 10⁴	2.38 x 10⁵	0.38

^*The errors associated with the data were <2% for ∆H and ∆H_v. The calorimetric heat (∆H) is the heat change per mole of enzyme. The van’t Hoff heat (∆H_v) is the heat change per cooperative unit. The ratio ∆H/∆H_v is a measure of the number of thermally transited cooperative units per mole of enzyme. All measurements were performed in triplicate.

ISSN: 1471-2180