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Figure 6 | BMC Microbiology

Figure 6

From: Stabilized homoserine o-succinyltransferases (MetA) or L-methionine partially recovers the growth defect in Escherichia coli lacking ATP-dependent proteases or the DnaK chaperone

Figure 6

In vitro degradation of the native MetA protein and stabilized I229Y mutant by the ATP-dependent proteases Lon, ClpP/X and HslVU. Degradation reactions were performed at 37°C with or without ATP as described in the Methods section. Untreated proteins indicate the positions of native MetA (the central lane of the upper gel) and mutant I229Y (the left lane of the lower gel). Densitometry results were normalized after setting the MetA amount before ATP addition equal to 100%. The results are plotted as the mean and standard deviation of two independent experiments.

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