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Figure 5 | BMC Microbiology

Figure 5

From: Stabilized homoserine o-succinyltransferases (MetA) or L-methionine partially recovers the growth defect in Escherichia coli lacking ATP-dependent proteases or the DnaK chaperone

Figure 5

L-methionine stimulates growth of Δ dnaK or protease-deficient mutants of the E. coli strain WE at non-permissive temperatures. The strains were cultured in 25 ml of M9 glucose medium with or without L-methionine supplementation (50 μg/ml) in 125 ml Erlenmeyer flasks at 37°C (∆dnaK mutants) or 42°C (protease-minus mutants). The average of two independent experiments is presented. Serial dilutions of logarithmically growing at 30°C (∆dnaK mutants) or 37°C (protease-minus mutants) in M9 glucose medium cultures (OD600 of 0.5) were spotted onto M9 glucose or M9 glucose L-methionine (50 μg/ml) agar plates. The cells were incubated for 24 h at 37°C (∆dnaK mutants) or 42°C (protease-minus mutants).

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BMC Microbiology

ISSN: 1471-2180

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