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Figure 3 | BMC Microbiology

Figure 3

From: Stabilized homoserine o-succinyltransferases (MetA) or L-methionine partially recovers the growth defect in Escherichia coli lacking ATP-dependent proteases or the DnaK chaperone

Figure 3

In vivo stability of MetA mutants. Cells of the strains WE, L124 and Y229 exponentially growing (OD600 = 0.3) at 37°C in M9 medium were treated with 200 μg/ml of chloramphenicol. The cultures were divided; one half of each culture was maintained at 37°C (solid symbols), and the other half of the culture was shifted to 44°C (open symbols). The samples were collected at the indicated time points and analyzed through Western blotting as described in the Methods section. Densitometry results were normalized after setting the MetA amount before chloramphenicol addition equal to 100%.

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BMC Microbiology

ISSN: 1471-2180

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