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Figure 2 | BMC Microbiology

Figure 2

From: Stabilized homoserine o-succinyltransferases (MetA) or L-methionine partially recovers the growth defect in Escherichia coli lacking ATP-dependent proteases or the DnaK chaperone

Figure 2

Heat-induced aggregation of native and mutant MetAs in vitro . Aggregated proteins were prepared through incubation at 45°C for 30 min as described in the Methods section; the soluble (black columns) and insoluble (gray columns) protein fractions were separated by centrifugation at 14,000 g for 30 min and analyzed through Western blotting with rabbit anti-MetA antibodies. The densitometric analysis of band intensity was conducted using WCIF Image J software. The total amount of MetAs before an incubation was equal to 1. The error bars represent the standard deviations of duplicate independent cultures.

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