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Table 2 Genotypes identified in PCR positive swabs and whole blood samples

From: Comparison of CDC and sequence-based molecular typing of syphilis treponemes: tpr and arp loci are variable in multiple samples from the same patient

Genes

 

Type of sample

Statistical significance

 

Locus nucleotide sequences

Swabs (n = 93)

WB samples (n = 34)

 

TP0136

Identical to strain SS14

96.1% (74/77)

100.0% (12/12)

 
 

Unique sequences

3.9% (3/77)

0.0% (0/12)

 

TP0548

Identical to strain SS14

74.4% (58/78)

68.8% (11/16)

 
 

Unique sequences

25.6% (20/78)

31.3% (5/16)

 

23S rDNA

wt

60.4% (55/91)

51.6% (16/31)

 
 

A2058G

28.6% (26/91)

25.8% (8/31)

 
 

A2059G

11.0% (10/91)

22.6% (7/31)

 
 

Number of repetitions in the arp locus

  
  

Swabs

WB samples

 

arp

14

82.4% (56/68)

55.6% (5/9)

p = 0.03

 

15

11.8% (8/68)

11.1% (1/9)

 
 

8-12

5.9% (4/68)

33.3% (3/9)

p = 0.003

tpr E, G, J

tpr E, G, J pattern after Mse I digest

   
  

Swabs

WB samples

 
 

d

91.2% (62/68)

30.8% (4/13)

p < 0.001

 

e

1.5% (1/68)

46.2% (6/13)

p < 0.001

 

b, p, k, j

7.4% (5/68)

23.1% (3/13)

 
  1. Samples isolated in the work of Flasarová et al. [17] augmented by samples collected in 2011 in the Czech Republic were analyzed. Results show both paired and unpaired samples.
  2. wt, wildtype.