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Table 1 Nucleotide sequence changes in the rfbT gene of different Inaba strains of V. cholerae O1 El Tor relative to El Tor Ogawa strain B33

From: Sequence polymorphisms of rfbT among the Vibrio cholerae O1 strains in the Ogawa and Inaba serotype shifts

Mutational event

Change(s)

Strain(s)

Consequence

Transposase insertion

Transposase inserted at 5′ region of rfbT

N16961, IEC224, LMA3984-4, GX06002*, SD95001*

RfbT inactivation

Single nucleotide mutation

G-3 to A

ZJ011319*, GD01049, CQ02057, GX01012, JX01002, LN01-1, CQ01008

114 amino acids of N-terminal untranslated

A-20 deleted

ZJ05070

Frameshift: K6NYVQKL to K6TMYKN-Stop

A inserted after G-55

XJ7517, XJ81759*

Frameshift: D18AIQSKSVHD to D18 DYSVKVCS-Stop

G −137 to T

569B§, 16510§, 16186§, 16177§, 16159§, 16156§, 16148§, 1119§, 16020§, 16002§, 16121§, 16505§, 16507§, NIH35A3§

RfbT W46 to L

T-303 deleted

569B§, 1119§, 16020§, 16002§, 16505§, 16507§

RfbT T102 to H

C inserted after C-307

569B§, 1119§, 16020§, 16002§, 16505§, 16507§

16121*§, 16148§, 16510§, 16186§, 16177§, 16159§, 16156§, NIH35A3§

Frameshift: T102QGKIIAIEPLTEMENS to T102PRKNYSDRTTHRNGK-Stop

C-307 to T

E506

Frameshift: T102Q to T102-Stop

T-472 to C

FJ05234*, ZJ05023, GD05039, HL08091, FJ147, CIRS101

RfbT S158 to P

A-482 to T

XJ05021*

RfbT Y161 to F

C-487 to A

569B§, 16510§, 16186§, 16177§, 16159§, 16156§, 16148§, 1119§, 16020§, 16002§, 16121§, 16505§, 16507§, NIH35A3§

RfbT Q163 to K

A-494 deleted

16020*§, 16002§, 16505§, 16507§, 16121§, 1119§; GX00107*, XJ05021*, GD91070, GD06119, GD06009, JX04043, GX06021

Frameshift: K164NTDI to K164IQT-Stop (16121 yields truncated proteins due to nonsense mutations at 307nt)

A-495 deleted

V01, C6706, X190

Frameshift: K164NTDI to K164IQT-Stop

C-563 to A

TJ64600, JS32*, BJ6588,

Frameshift: I187S to I187-Stop

G-655 to T

569B§

Frameshift: N218G to N218-Stop

C-785 to A

LN04060

Frameshift: L261S to L261-Stop

Multiple nucleotides mutation

TGATGCT inserted after T-57

MJ1236

Frameshift: A19I to A19-Stop

TATG insterted after G-207

T21

Frameshift: M69RHWIVNH to M69YASLDC-Stop

GACACAT inserted after T-237

HE65441*, BJ84203, JX8672, JS63257

Frameshift: T81T to T81-Stop

GCTGAACATCC deleted after C-789

BJ821*, JX801361, BJ83801, FJ85063, HN8232, JS80269, JX801360, SD83101, AH88602, FJ86104, HN81331, JX801309, JX801363, BJ83795, JS80215, JX801305, JX84172, JX8788, SD83163, SD83164, SD83167, SX8429, JX801342, HN84345, FJ80004, GD791080, GD791084, GD861812, HN81175, JX801295, JX8659, JX87123, SC83535, ZJ861071, FJ8004, JX84190, LN85092, SD83176, JX801290, JS80252, FJ85010, ZJ82428

Frameshift: S263A to S263-Stop

  1. * the strain selected as the representative of the different kinds of mutations and examined the seroconversion in complement assay by inducing a rfbT- expressing plasmid, pBR-rfbT.
  2. § classical biotype.