Characterization of the PAS domain in the sensor-kinase BvgS: mechanical role in signal transmission

BMC Microbiology

Table 1 Relevant features of the proteins produced in this work

Name	Residue range*	Calculated MW (Da)^#	Tm (°C)
PAS core	592-697	13,193	nd
N1C1	566-701	16,960	nd
N1C2	566-714	18,438	nd
N1C3	566-720	19,049	nd
N2C1	573-701	15,994	nd
N2C2	573-714	17,472	69.7 ± 0.2
N2C3	573-720	18,083	70.5 ± 0.3
N3C1	581-701	15,096	nd
N3C2	581-714	16,574	63.1 ± 0.2
N3C3	581-720	17,185	61.1 ± 0.5
Y₅₉₆A + N₆₃₁A	573-720	17,948	nd
C₆₀₇A	573-720	18,051	62.3 ± 0.2
N₆₀₈A	573-720	18,040	nd
N₆₀₈S	573-720	18,056	60.3 ± 0.6
H₆₄₃A	573-720	18,017	63.0 ± 0.4
R₆₇₀A	573-720	17,998	66.8 ± 0.2
D₆₉₅A	573-720	18,039	60.1 ± 0.1

* The numbering refers to full-length BvgS starting from the initiation Met residue.
# The calculated molecular masses (for a monomer) comprise the start linker from the pASK plasmid without the initiation Met (ASRGSHHHHHHGA). For the PAS core the start linker sequence is RGSHHHHHHGS.
nd, not determined (see text).
The Tms of the N2C3 variants were all significantly different (P < 0.01) from that of the wt N2C3 protein.

ISSN: 1471-2180