Sub-inhibitory concentrations of some antibiotics can drive diversification of Pseudomonas aeruginosa populations in artificial sputum medium

Table 3 Oligonucleotide primers used in this study

Name	Sequence (5′-3′)	Size (bp)	Annealing temperature (°C)	Target gene	Reference
LESD3cIF	ATGAAAAAGCCCGTAAGA	490	55	LES prophage 5 c I repressor gene	[13]
LESD3cIR	GCCATTCCCGCTTAAAAG	490	55	LES prophage 5 c I repressor gene	[13]
LES1F	TCGGCGTAATGTCCTCTA	392	68	LES prophage 2	[59]
LES1R	TGAAGCCGACGATGGAAG	392	68	LES prophage 2	[59]
PS1F	ACAGAATATTCGAAGCAG	338	58	LES genomic island-5	[59]
PS1R	ACAAGAGCCTAACACCAC	338	58	LES genomic island-5	[59]

ISSN: 1471-2180