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Table 3 Oligonucleotide primers used in this study

From: Sub-inhibitory concentrations of some antibiotics can drive diversification of Pseudomonas aeruginosa populations in artificial sputum medium

Name Sequence (5′-3′) Size (bp) Annealing temperature (°C) Target gene Reference
LESD3cIF ATGAAAAAGCCCGTAAGA 490 55 LES prophage 5 c I repressor gene [13]
LESD3cIR GCCATTCCCGCTTAAAAG
LES1F TCGGCGTAATGTCCTCTA 392 68 LES prophage 2 [59]
LES1R TGAAGCCGACGATGGAAG
PS1F ACAGAATATTCGAAGCAG 338 58 LES genomic island-5 [59]
PS1R ACAAGAGCCTAACACCAC