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Table 3 Abundances of 13 C in P(3HB) synthesized by R. eutropha H16 and cbbLS disruptants on fructose with addition of NaH 13 CO 3 a

From: Detection of phase-dependent transcriptomic changes and Rubisco-mediated CO2 fixation into poly (3-hydroxybutyrate) under heterotrophic condition in Ralstonia eutropha H16 based on RNA-seq and gene deletion analyses

R. eutropha strain NaHCO3addedb P(3HB) (wt%) 13C-Abundance in P(3HB)c(%) Increase of 13C in P(3HB) (mmol/g-P(3HB))
H16 12C 53.6 ± 2.14 1.13 ± 0.0003 -
  13C 49.5 ± 4.39 2.22 ± 0.0025 0.42 ± 0.0016
H16∆cbbLS c 12C 52.1 ± 0.91 1.10 ± 0.0001 -
  13C 48.3 ± 1.41 1.81 ± 0.0013 0.27 ± 0.0007
H16∆cbbLS p 12C 50.0 ± 2.49 1.13 ± 0.0002 -
  13C 48.3 ± 2.48 2.11 ± 0.0022 0.38 ± 0.0012
H16∆∆cbbLS 12C 27.8 ± 0.17 1.11 ± 0.0003 -
  13C 30.0 ± 0.48 1.25 ± 0.0005 0.05 ± 0.0004
  1. aP(3HB) biosynthesis was performed by 2-stage cultivation as described in the text.
  2. bAdded periodically during the second stage.
  3. cMeans of 13C/12C ratios calculated from isotopomer abundances of the three fragments (m/z 45, 87, and 103) derived from 3HB methyl ester.