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Table 3 Abundances of 13 C in P(3HB) synthesized by R. eutropha H16 and cbbLS disruptants on fructose with addition of NaH 13 CO 3 a

From: Detection of phase-dependent transcriptomic changes and Rubisco-mediated CO2 fixation into poly (3-hydroxybutyrate) under heterotrophic condition in Ralstonia eutropha H16 based on RNA-seq and gene deletion analyses

R. eutropha strain

NaHCO3addedb

P(3HB) (wt%)

13C-Abundance in P(3HB)c(%)

Increase of 13C in P(3HB) (mmol/g-P(3HB))

H16

12C

53.6 ± 2.14

1.13 ± 0.0003

-

 

13C

49.5 ± 4.39

2.22 ± 0.0025

0.42 ± 0.0016

H16∆cbbLS c

12C

52.1 ± 0.91

1.10 ± 0.0001

-

 

13C

48.3 ± 1.41

1.81 ± 0.0013

0.27 ± 0.0007

H16∆cbbLS p

12C

50.0 ± 2.49

1.13 ± 0.0002

-

 

13C

48.3 ± 2.48

2.11 ± 0.0022

0.38 ± 0.0012

H16∆∆cbbLS

12C

27.8 ± 0.17

1.11 ± 0.0003

-

 

13C

30.0 ± 0.48

1.25 ± 0.0005

0.05 ± 0.0004

  1. aP(3HB) biosynthesis was performed by 2-stage cultivation as described in the text.
  2. bAdded periodically during the second stage.
  3. cMeans of 13C/12C ratios calculated from isotopomer abundances of the three fragments (m/z 45, 87, and 103) derived from 3HB methyl ester.