Detection of phase-dependent transcriptomic changes and Rubisco-mediated CO2 fixation into poly (3-hydroxybutyrate) under heterotrophic condition in Ralstonia eutropha H16 based on RNA-seq and gene deletion analyses

Table 2 Highly transcribed clusters in R. eutropha H16 during cultivation on fructose

Clusters^a	Gene IDs	Representative products or functions	Highly transcribed phase(s)
A	H16_A0976-A0993	Pilus assembly proteins	Growth
B	H16_A1047-A1063	NADH dehydrogenase subunits, triosephosphate isomerase TpiA	Growth
C	H16_A2305-A2321	Translation initiation factor InfB, transcription elongation factor NusA, cytchrome c oxisdase subunits	Growth
D	H16_A2359-A2369	RNA-binding protein Hfq, GTP-binding protein EngA, histidyl-tRNA synthetase, nucleoside diphosphate kinase	Growth
E	H16_A2560-A2572	Sigma factor RpoE, sigma E-negative regulatory proteins, fatty acid biosynthesis	Growth
F	H16_A2889-A2905	Cell wall biogenesis	Growth
G	H16_A3268-A3282	Cell division proteins, peptidoglycan biosynthesis	Growth
H	H16_A3457-A3484	Ribosomal proteins, RNA polymerase subunit α, translation initiation factor InfA	Growth, PHA production, Stationary
I	H16_A3490-A3505	Ribosomal proteins, elongation factors, RNA polymerase subunits ββ’, transcription antiterminator NusG	Growth, PHA production, Stationary
J	H16_A3636-A3643	F₀F₁ ATP synthase subunits	Growth
K	H16_A1949-A1957	Metylmalonyl-CoA mutase, K⁺ transport flavoprotein	PHA production
L	H16_B1380-B1395	Calvin-Benson-Bassham cycle	PHA production
M	H16_B1497-B1503	ABC-type fructose transporter, Entner-Doudoroff pathway	Growth
N	PHG001-PHG023	Membrane-bound hydrogenase subunits, hydrogenase accessory proteins	Growth, PHA production, Stationary
O	PHG088-PHG096	Soluble hydrogenase subunits, hydrogenase accessory proteins	Growth, Stationary
P	PHG416-PHG427	Calvin-Benson-Bassham cycle	PHA production

ISSN: 1471-2180