Table 3 Compilation of primers used for the amplification of ITS, EF-1a, ß-tubulin, RPB2, BotF15 of the fungus, and of partial 16S rDNA region of the bacteria
Forward primer 5′ – 3′ | Reverse primer 5′ – 3′ | Literature | |
|---|---|---|---|
ITS | ITS1: TCCGTAGGTGAACCTGCGG | ITS4: TCCTCCGCTTATTGATATGC | White et al. 1990 [15] |
EF-1a | EF-AF: CATCGAGAAGTTCGAGAAGG | EF-BR: CRAT GGT GAT ACC RCG CTC | Pavlic et al. 2009 [18] |
ß-tubulin | Bt2a: GGTAACCAAATCGGTGCTGCTTTC | Bt2b: ACCCTCAGTGTAGTGACCCTTGGC | Pavlic et al. 2009 [18] |
RPB2 | RPB2bot6F: GGTAGCGACGTCACTCCC | RPB2bot7R: GGATGGATCTCGCAATGCG | Pavlic et al. 2009 [18] |
BotF15 | Bot15: CTGACTTGTGACGCCGGCTC | Bot16: CAACCTGCTCAGCAAGCGAC | Pavlic et al. 2009 [18] |
16S rDNA | 27 F: AGAGTTTGATGCTCAG | 765R: CTGTTTGCTCCCCACGGTTTC | Coombs and Franco 2003 [33] |