Analysis of HC-toxin from A. jesenskae by HPLC and TLC. (A) HPLC of standard HC-toxin (10 μg). (B) HPLC of A. jesenskae culture filtrate extracted with dichloromethane (400 μl equivalent crude culture filtrate). Detection in both cases was at 230 nm. (C) TLC of (1) native HC-toxin, and (2) material from A. jesenskae eluting between 8 and 10 min from HPLC of the separation shown in panel B. Visualization used an epoxide-specific reagent . The asterisk indicates the position of HC-toxin.