The new species Enterobacter oryziphilus sp. nov. and Enterobacter oryzendophyticus sp. nov. are key inhabitants of the endosphere of rice

Table 2 Key reactions for biochemical differentiation of selected Enterobacter species

Characteristic^a	1	2	3	4	5	6	7	8
Voges-Proskauer test (37°C)^b	+	+	+	-	-	-	+	+
Methyl red test	+	+	+	+	+	+	-	-
Cell morphology	SR	SR	R	CR	CR	CR	R	R
Ornithine decarboxylase^b	-	+	-	-	-	-	+	+
Malonate decarboxylase (48 h)	+	+	+	-	+	+	-	+
Arginine dehydrolase^b	+	+	+	-	-	-	+	+
Esculin hydrolysis	+	+	+	-	-	+	-	+
Citrate^b	+	+	+	+	-	-	+	+
Gluconate dehydrogenase	+	+	+	-	+	+	-	ND
Carbon source utilization^c
Sucrose	+	+	+	-	-	+	+	+
D-melibiose	-	V	-	+	+	+	+	+
Adonitol	+	+	-	-	-	-	-	-
D-sorbitol	+	+	+	-	-	-	+	+
L-fucose	+	V	ND	-	-	-	-	-
L-aspartic acid	+	-	+	+	+	+	ND	ND
m-inositol	+	+	-	-	-	-	+	+
D-arabitol	+	+	-	-	-	+	-	-
D-raffinose	-	+	-	-	-	+	+	+

^aFor all strains analysed, the following tests were positive: catalase, β-galactosidase (ONPG) and motility and negative for: oxidase, lysine decarboxylase, urease, indole and H₂S production.
^bTest results of both the API-20E system and conventional test methods.
^cThe carbon source utilization tests were determined by using Biolog GN2 microplates.
*Species: 1, Enterobacter oryziphilus sp. nov. (n=3); 2, Enterobacter oryzendophyticus sp. nov. (n=3); 3, Enterobacter radicincitans D5/23^T; 4, Enterobacter turicensis 508/05^T; 5, Enterobacter helveticus 513/05^T; 6, Enterobacter pulveris 601/05^T. 7, Enterobacter cloacae subsp. cloacae; data from [23–25]; 8, Enterobacter cloacae subsp. dissolvens, data from [8, 26]. The percentage of strains giving a positive result is scored as: -, 0–20%; V, 20–80%; +, 80–100%; ND, no data available; cell morphology: R, rods; CR, coccoid rods; SR, straight rods.

ISSN: 1471-2180