Figure 5

Proposed Mechanism for Redox-Sensitive Transcriptional Regulation by MaMsvR. EMSA experiment with pre-reduced MaMsvR and various treatments. The P _msvR DNA (10 nM) only control reaction is represented by (-). All other lanes contain P _msvR DNA (10 nM) and 200 nM MaMsvR^Pre-Red either in the absence (+, O) or presence (R, OR) of 5 mM DTT. Lanes labeled with (O) also contain 10 μM H₂O₂.