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Table 1 Identification of clinical isolates (n=158) by conventional methods compared to 16S rRNA gene sequence analysis

From: Accurate identification of fastidious Gram-negative rods: integration of both conventional phenotypic methods and 16S rRNA gene analysis

Conventional phenotyic methods

 

16S rRNA gene sequence analysis

  

Final identification (supplemental conventional tests if required)

Identification (number of isolates)

Level of identification and correctness of result

Best reference species sequence

% difference to reference species sequence

GenBank accession numbers

 

Actinobacillus ureae (1)

S 1; SI 2

Actinobacillus hominis Actinobacillus suis (low demarcation)

0.0, 0.4

KC866152

A. hominis (acidification of mannitol: A. hominis (positive), A. suis (negative) [1])

Aggregatibacter actinomycetemcomitans (2)

S; SC

Aggregatibacter actinomycetemcomitans

0.0, 0.3

KC866227; KC866228

A. actinomycetemcomitans

Aggregatibacter actinomycetemcomitans (1)

S; SI

Pasteurella bettyae

0.0

KC866143

P. bettyae

Aggregatibacter aphrophilus (11)

S; SC

Aggregatibacter aphrophilus

0.0-0.8

KC866144; KC866145; KC866146; KC866147; KC866148; KC866149; KC866150; KC866229; KC866230; KC866231; KC866272

A. aphrophilus

Aggregatibacter aphrophilus (2)

S; SI

Aggregatibacter aphrophilus

3.8, 2.9

KC866151; KC866153

Aggregatibacter sp.

Aggregatibacter aphrophilus (1)

S; SI

Neisseria sicca

0.8

KC866154

N. sicca (nitrate reduction: positive (N. mucosa), negative (N. sicca, N. subflava bv. flava); sucrose acidification: positive (N. sicca, N. mucosa), negative (N. subflava bv. flava) [18])

Neisseria subflava bv. flava

1.0

Neisseria mucosa (low demarcation)

1.1

Aggregatibacter sp. (1)

G; GC

Aggregatibacter aphrophilus

2.3

KC866155

Aggregatibacter sp.

Bergeyella zoohelcum (1)

S; SI

Myroides odoratimimus

5.9

KC866156

Flavobacteriaceae

Bergeyella zoohelcum (1)

S; SI

Neisseria zoodegmatis

0.3

KC866157

N. zoodegmatis

Capnocytophaga canimorsus (2)

S; SC

Capnocytophaga canimorsus

0.5, 0.4

KC866158; KC866159

C. canimorsus

Capnocytophaga ochracea (1)

S; SI

Capnocytophaga gingivalis

0.6

KC866160

C. gingivalis

Capnocytophaga ochracea (1)

S; SI

Capnocytophaga ochracea

2.5

KC866161

Capnocytophaga sp.

Capnocytophaga ochracea (5)

S; SI

Capnocytophaga sputigena

0.0-0.3

KC866162; KC866163; KC866164; KC866273; KC866274

C. sputigena 3

Capnocytophaga ochracea (1)

S; SI

Dysgonomonas mossii

0.6

KC866165

D. mossii

Capnocytophaga ochracea (1)

S; SI

Leptotrichia trevisanii

0.2

KC866166

L. trevisanii

Capnocytophaga sp. (2)

G; GC

Capnocytophaga sputigena

0.0, 0.6

KC866167; KC866232

C. sputigena

Cardiobacterium hominis (4)

S; SC

Cardiobacterium hominis

0.0-0.5

KC866168; KC866233; KC866275; KC866299

C. hominis

CDC Group IIe (1)

S; SI

Chryseobacterium anthropi

0.2

KC866169

C. anthropi (acidification of fructose and sucrose: positive (C. haifense), negative (C. anthropi) [19])

Chryseobacterium haifense (low demarcation)

0.6

Comamonas sp. (1)

G; GI

Oligella urethralis

0.0

KC866170

O. urethralis

Dysgonomonas capnocytophagoides (1)

S; SC

Dysgonomonas capnocytophagoides

0.2

KC866171

D. capnocytophagoides

Eikenella corrodens (10)

S; SC

Eikenella corrodens

0.0-0.8

KC866172; KC866173; KC866174; KC866175; KC866176; KC866177; KC866178; KC866234; KC866235; KC866236

E. corrodens

Flavobacterium sp. (1)

G; GC

Flavobacterium lindanitolerans

0.4

KC866179

F. lindanitolerans

Gram-negative rods (1)

N

Actinobacillus hominis

0.3

KC866238

A. hominis

Gram-negative rods (1)

N

Actinobacillus hominis

0.0

KC866237

A. hominis (esculin hydrolysis: positive (A. suis), variable (A. hominis), negative (A. equuli); mannitol acidification: positive (A. equuli, A. hominis), negative (A. suis) [1])

Actinobacillus suis

0.0

Actinobacillus equuli (low demarcation)

0.5

Gram-negative rods (1)

N

Aggregatibacter actinomycetemcomitans

0.2

KC866239

A. actinomycetemcomitans

Gram-negative rods (2)

N

Aggregatibacter aphrophilus

0.3, 0.8

KC866240; KC866241

A. aphrophilus

Gram-negative rods (1)

N

Azospira oryzae

0.0

KC866276

A. oryzae

Gram-negative rods (1)

N

Brevundimonas terrae

0.6

KC866180

B. terrae

Gram-negative rods (3)

N

Capnocytophaga canimorsus

0.0-0.2

KC866277; KC866278; KC866279

C. canimorsus

Gram-negative rods (1)

N

Capnocytophaga sputigena

0.0

KC866280

C. sputigena

Gram-negative rods (2)

N

Cardiobacterium hominis

0.5, 0.6

KC866281; KC866282

C. hominis

Gram-negative rods (1)

N

Chryseobacterium haifense

0.2

KC866181

C. anthropi (acidification of fructose and sucrose: positive (C. haifense), negative (C. anthropi) [19])

Chryseobacterium anthropi (low demarcation)

0.5

Gram-negative rods (1)

N

Kingella denitrificans

0.0

KC866182

K. denitrificans

Gram-negative rods (1)

N

Moraxella atlantae

0.2

KC866242

M. atlantae

Gram-negative rods (2)

N

Moraxella lacunata

0.0

KC866283; KC866284

M. lacunata

Gram-negative rods (1)

N

Moraxella lincolnii

0.3

KC866243

M. lincolnii

Gram-negative rods (3)

N

Moraxella nonliquefaciens

0.0-0.7

KC866285; KC866286; KC866287

M. nonliquefaciens

Gram-negative rods (2)

N

Moraxella osloensis

0.0, 0.2

KC866288; KC866289

M. osloensis

Gram-negative rods (1)

N

Neisseria bacilliformis

0.0

KC866244

N. bacilliformis

Gram-negative rods (1)

N

Neisseria zoodegmatis

2.0

KC866245

Neisseria sp.

Gram-negative rods (4)

N

Neisseria elongata

0.0-0.3

KC866246; KC866247; KC866290; KC866291

N. elongata

Gram-negative rods (1)

N

Neisseria flavescens

0.5

KC866248

N. subflava (acidification of glucose and maltose: positive (N. subflava), negative (N. flavescens) [18])

Neisseria subflava (low demarcation)

0.7

Gram-negative rods (2)

N

Neisseria flavescens

0.3

KC866249; KC866250

N. subflava (acidification of glucose and maltose: positive (N. subflava), negative (N. flavescens) [18])

Neisseria subflava (low demarcation)

0.4

Gram-negative rods (4)

N

Neisseria weaveri

0.0-0.3

KC866251; KC866252; KC866253; KC866254

N. weaveri

Gram-negative rods (1)

N

Pasteurella bettyae

0.0

KC866292

P. bettyae

Gram-negative rods (1)

N

Pasteurella dagmatis

0.4

KC866255

P. stomatis (urease reaction: positive (P. dagmatis), negative (P. stomatis); acidification of maltose: positive (P. dagmatis), negative (P. stomatis) [1])

Pasteurella stomatis (low demarcation)

0.4

Kingella denitrificans (1)

S; SC

Kingella denitrificans

0.6

KC866183

K. denitrificans

Kingella denitrificans (1)

S; SI

Neisseria elongata

0.0

KC866184

N. elongata

Leptotrichia buccalis (1)

S; SI

Leptotrichia trevisanii

0.3

KC866293

L. trevisanii

Moraxella lacunata (1)

S; SC

Moraxella lacunata

0.5

KC866185

M. lacunata (gelatinase reaction: positive (M. lacunata), negative (M. nonliquefaciens) [20])

Moraxella nonliquefaciens (low demarcation)

0.7

Moraxella osloensis (1)

S; SC

Moraxella osloensis

0.0

KC866186

M. osloensis

Moraxella osloensis (1)

S; SI

Psychrobacter faecalis

0.0

KC866187

P. pulmonis (acidification of glucose and xylose: positive (P. faecalis), negative (P. pulmonis) [20])

Psychrobacter pulmonis (low demarcation)

0.2

Moraxella sp. (1)

G; GC

Moraxella canis

0.2

KC866188

M. canis

Neisseria sp. (1)

G; GI

Neisseria elongata

0.3

KC866256

N. elongata

Moraxella sp. (4)

G; GC

Moraxella nonliquefaciens

0.0-0.3

KC866189; KC866190; KC866257; KC866258

M. nonliquefaciens

Moraxella sp. (8)

G; GC

Moraxella osloensis

0.0-0.2

KC866191; KC866192; KC866193; KC866194; KC866259; KC866260; KC866261; KC866294

M. osloensis

Neisseria animaloris (EF4a) (1)

S; SC

Neisseria animaloris

0.0

KC866195

N. animaloris

Neisseria animaloris (EF4a) (1)

S; SI

Neisseria zoodegmatis

0.0

GU797849

N. zoodegmatis

Neisseria cinerea (2)

S; SC

Neisseria cinerea

0.0

KC866196; KC866197

N. cinerea (acidification of glucose and maltose: positive (N. meningitidis), negative (N. cinerea) [18])

Neisseria meningitidis (low demarcation)

0.3

Neisseria elongata (1)

S; SI

Aggregatibacter aphrophilus

2.4

KC866198

Aggregatibacter sp.

Neisseria elongata (3)

S; SC

Neisseria elongata

0.0-0.3

KC866203; KC866204; KC866205

N. elongata

Neisseria elongata (2)

S; SI

Neisseria bacilliformis

0.1, 0.4

KC866201; KC866202

N. bacilliformis

Neisseria elongata (1)

S; SI

Neisseria zoodegmatis

0.6

KC866206

N. zoodegmatis

Neisseria elongata (2)

S; SI

Eikenella corrodens

0.0

KC866199; KC866200

E. corrodens

Neisseria sp. (1)

G; GC

Neisseria shayeganii

0.3

KC866207

N. shayeganii

Neisseria sp. (1)

G; GC

Neisseria elongata

0.2

KC866270

N. elongata

Neisseria sp. (1)

G; GC

Neisseria oralis

0.0

KC866208

N. oralis

Neisseria weaveri (1)

S; SC

Neisseria weaveri

0.0

KC866211

N. weaveri

Neisseria weaveri (1)

S; SC

Neisseria shayeganii

0.2

KC866210

N. shayeganii

Neisseria weaveri (1)

S; SI

Azospira oryzae

0.0

KC866209

A. oryzae

Neisseria zoodegmatis (EF4b) (3)

S; SC

Neisseria zoodegmatis

0.0-0.5

KC866212; KC866213; KC866295

N. zoodegmatis

Oligella urethralis (2)

S; SC

Oligella urethralis

0.0

KC866214; KC866215

O. urethralis

Pasteurella aerogenes (1)

S; SI

Pasteurella aerogenes

2.7

KC866226

Pasteurella sp.

Pasteurella bettyae (2)

S; SC

Pasteurella bettyae

0.0

KC866216; KC866262

P. bettyae

Pasteurella canis (1)

S; SC

Pasteurella canis

0.0

KC866217

P. canis

Pasteurella canis (1)

S; SI

Pasteurella stomatis

1.6

KC866218

Pasteurella sp.

Pasteurella dagmatis (1)

S; SC

Pasteurella dagmatis

0.2

KC866271

P. dagmatis

Pasteurella multocida (14)

S; SC

Pasteurella multocida

0.0-0.2

KC866219; KC866220; KC866221; KC866222; KC866223; KC866263; KC866264; KC866265; KC866266; KC866267; KC866268; KC866296; KC866297; KC866298

P. multocida

Pasteurella pneumotropica (1)

S; SI

Bisgaard Taxon 22

1.7

KC866224

Pasteurella sp.

Pasteurella sp. (1)

G; GI

Necropsobacter rosorum

0.0

KC866269

N. rosorum

Roseomonas sp. (1)

G; GC

Roseomonas mucosa

0.0

KC866225

R. mucosa

  1. 1Assignment to taxonomic level: S = species, G = genus, N = not identified.
  2. 2Correctness of assignment: SC = correct at species level, SI = incorrect at species level, GC = correct at genus level, GI = incorrect at genus level, N = not identified.
  3. 3 Difficult differentiation of species in question by conventional tests.