Table 4 Growth media used in this work

RPMI

8.4 g L^-1 RPMI 1640 (Sigma-Aldrich R1383), 2 g L^-1 glucose, 0.165 M 3-(N-morpholino propanesulfonic acid (MOPS), adjusted to pH 7.3 with 10 N NaOH

YNB

6.7 g L^-1 Yeast Nitrogene Base (Sigma Y1250), 2 g L^-1 glucose, 0.165 M 3-(N-morpholino propanesulfonic acid (MOPS), adjusted to pH 7.3 with 10 N NaOH

YPD

Sufficient iron medium: Yeast extract (10 g L^-1) peptone (20 g L^-1) dextrose (20 g L^-1) (Sigma-Aldrich Y1375)

RIM

Restricted iron medium; YPD + 200 μM bathophenantroline disulfunate (BPS) (Sigma 146617)