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Table 4 Growth media used in this work
| Medium | Composition |
|---|---|
| RPMI | 8.4 g L-1 RPMI 1640 (Sigma-Aldrich R1383), 2 g L-1 glucose, 0.165 M 3-(N-morpholino propanesulfonic acid (MOPS), adjusted to pH 7.3 with 10 N NaOH |
| YNB | 6.7 g L-1 Yeast Nitrogene Base (Sigma Y1250), 2 g L-1 glucose, 0.165 M 3-(N-morpholino propanesulfonic acid (MOPS), adjusted to pH 7.3 with 10 N NaOH |
| YPD | Sufficient iron medium: Yeast extract (10 g L-1) peptone (20 g L-1) dextrose (20 g L-1) (Sigma-Aldrich Y1375) |
| RIM | Restricted iron medium; YPD + 200 μM bathophenantroline disulfunate (BPS) (Sigma 146617) |
