Strain/plasmids | Relevant genotype/ phenotype | Reference |
---|---|---|
E. coli S17-1 | thi pro hsdR-M+, chromosomally integrated [RP4-2 Tc::Mu:Kmr::Tn7, Tra+ Trir Strr] | [65] |
P. aeruginosa | Â | [38] |
PABST7.1/pUCPL6A | Overexpression of lipA and lipH from pUCPL6A | |
FRD1 | Mucoid ΔmucA22 CF-lung isolate | [66] |
FRD1153 | ΔalgJ5-mutant derived from FRD1, defect in O-acetylation of alginate | |
SG81 | Mucoid biofilm isolate from technical water system | [67] |
SG81MCS | Vector control pBBR1MCS | [1] |
SG81ΔlipA | Δ(2/3 lipA 1/5 lipH)::Ω-Gmr , deletion of lipA and lipH | This study |
SG81ΔlipA::lipA | Deletion of lipA and lipH complemented in trans from pBBL7 | This study |
SG81lipA+ | Expression of lipA and lipH in trans from pBBL7 | [1] |
pBBR1MCS | lacZ α Cmr mob Plac, PT7 | [64] |
pBBL7 | 2.8 kbp Xmn I/Sma I fragment with lipA/H operon in pBBR1MCS under Plac control | Â |
pMEΔAH11 | 2.06 kbp Kpn I/Xba I-fragment with Δ(2/3 lipA 1/5 lipH) in pME3087 | [63] |
pMEΔAH::Ω-Gmr | 1.6 kbp Sma I-fragment with Ω-Gmr from pBSL142 in pMEΔAH11 | This study |