Skip to main content

Advertisement

Table 3 Bacterial strains and plasmids used in this study

From: Interaction between extracellular lipase LipA and the polysaccharide alginate of Pseudomonas aeruginosa

Strain/plasmids Relevant genotype/ phenotype Reference
E. coli S17-1 thi pro hsdR-M+, chromosomally integrated [RP4-2 Tc::Mu:Kmr::Tn7, Tra+ Trir Strr] [65]
P. aeruginosa   [38]
PABST7.1/pUCPL6A Overexpression of lipA and lipH from pUCPL6A
FRD1 Mucoid ΔmucA22 CF-lung isolate [66]
FRD1153 ΔalgJ5-mutant derived from FRD1, defect in O-acetylation of alginate [61, 62]
SG81 Mucoid biofilm isolate from technical water system [67]
SG81MCS Vector control pBBR1MCS [1]
SG81ΔlipA Δ(2/3 lipA 1/5 lipH)::Ω-Gmr , deletion of lipA and lipH This study
SG81ΔlipA::lipA Deletion of lipA and lipH complemented in trans from pBBL7 This study
SG81lipA+ Expression of lipA and lipH in trans from pBBL7 [1]
pBBR1MCS lacZ α Cmr mob Plac, PT7 [64]
pBBL7 2.8 kbp Xmn I/Sma I fragment with lipA/H operon in pBBR1MCS under Plac control  
pMEΔAH11 2.06 kbp Kpn I/Xba I-fragment with Δ(2/3 lipA 1/5 lipH) in pME3087 [63]
pMEΔAH::Ω-Gmr 1.6 kbp Sma I-fragment with Ω-Gmr from pBSL142 in pMEΔAH11 This study