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Table 3 Bacterial strains and plasmids used in this study

From: Interaction between extracellular lipase LipA and the polysaccharide alginate of Pseudomonas aeruginosa

Strain/plasmids

Relevant genotype/ phenotype

Reference

E. coli S17-1

thi pro hsdR-M+, chromosomally integrated [RP4-2 Tc::Mu:Kmr::Tn7, Tra+ Trir Strr]

[65]

P. aeruginosa

 

[38]

PABST7.1/pUCPL6A

Overexpression of lipA and lipH from pUCPL6A

FRD1

Mucoid ΔmucA22 CF-lung isolate

[66]

FRD1153

ΔalgJ5-mutant derived from FRD1, defect in O-acetylation of alginate

[61, 62]

SG81

Mucoid biofilm isolate from technical water system

[67]

SG81MCS

Vector control pBBR1MCS

[1]

SG81ΔlipA

Δ(2/3 lipA 1/5 lipH)::Ω-Gmr , deletion of lipA and lipH

This study

SG81ΔlipA::lipA

Deletion of lipA and lipH complemented in trans from pBBL7

This study

SG81lipA+

Expression of lipA and lipH in trans from pBBL7

[1]

pBBR1MCS

lacZ α Cmr mob Plac, PT7

[64]

pBBL7

2.8 kbp Xmn I/Sma I fragment with lipA/H operon in pBBR1MCS under Plac control

 

pMEΔAH11

2.06 kbp Kpn I/Xba I-fragment with Δ(2/3 lipA 1/5 lipH) in pME3087

[63]

pMEΔAH::Ω-Gmr

1.6 kbp Sma I-fragment with Ω-Gmr from pBSL142 in pMEΔAH11

This study