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Table 2 Inactivation temperatures of lipase LipA calculated by extrapolation of the linear gradient of the heat inactivation curves

From: Interaction between extracellular lipase LipA and the polysaccharide alginate of Pseudomonas aeruginosa

Sample T100 T50 T0
(°C) (°C) (°C)
Tris–HCl buffer (control) 45.0 +/− 2.5 63.8 +/− 1.1 82.7 +/− 2.9
Alginate FRD1 45.1 +/− 3.5 72.2 +/− 2.6 101.7 +/− 2.8
Alginate FRD1153 47.3 +/− 2.2 76.7 +/− 1.2 106.2 +/− 3.2
Alginate SG81 47.9 +/− 2.5 70.3 +/− 3.3 91.0 +/− 3.0
Alginate SG81, deacetylated 49.2 +/− 3.5 78.5 +/− 1.9 109.0 +/− 3.0
Algal alginate 54.0 +/− 4.7 68.1 +/− 2.7 87.2 +/− 3.4
Dextran 46.1 +/− 3.2 66.1 +/− 3.2 86.2 +/− 3.4
Xanthan 47.8 +/− 3.9 74.1 +/− 1.5 95.4 +/− 2.7
  1. The lipase activity was detected after 20 min incubation at different temperatures in the presence (1 mg/ml) and absence of polysaccharides. Three independent experiments were performed in duplicates. Shown are T0 representing the temperature of complete inactivation of lipase activity, T50 which represents the temperature at which the lipase activity was reduced by half and T100 designated the maximum temperature where lipase activity remained unaffected within 20 min of incubation.