Interaction between extracellular lipase LipA and the polysaccharide alginate of Pseudomonas aeruginosa

BMC Microbiology

Table 2 Inactivation temperatures of lipase LipA calculated by extrapolation of the linear gradient of the heat inactivation curves

Sample	T₁₀₀	T₅₀	T₀
Sample	(°C)	(°C)	(°C)
Tris–HCl buffer (control)	45.0 +/− 2.5	63.8 +/− 1.1	82.7 +/− 2.9
Alginate FRD1	45.1 +/− 3.5	72.2 +/− 2.6	101.7 +/− 2.8
Alginate FRD1153	47.3 +/− 2.2	76.7 +/− 1.2	106.2 +/− 3.2
Alginate SG81	47.9 +/− 2.5	70.3 +/− 3.3	91.0 +/− 3.0
Alginate SG81, deacetylated	49.2 +/− 3.5	78.5 +/− 1.9	109.0 +/− 3.0
Algal alginate	54.0 +/− 4.7	68.1 +/− 2.7	87.2 +/− 3.4
Dextran	46.1 +/− 3.2	66.1 +/− 3.2	86.2 +/− 3.4
Xanthan	47.8 +/− 3.9	74.1 +/− 1.5	95.4 +/− 2.7

The lipase activity was detected after 20 min incubation at different temperatures in the presence (1 mg/ml) and absence of polysaccharides. Three independent experiments were performed in duplicates. Shown are T₀ representing the temperature of complete inactivation of lipase activity, T₅₀ which represents the temperature at which the lipase activity was reduced by half and T₁₀₀ designated the maximum temperature where lipase activity remained unaffected within 20 min of incubation.

ISSN: 1471-2180