Figure 5

Proteolytic degradation of lipase LipA through endogenous LasB. Purified lipase LipA (18 ng/ml) from P. aeruginosa was incubated for 24 h at 37°C with 0.5 U purified LasB from P. aeruginosa (EMD4 Bioscience) in the absence and in the presence of bacterial alginate from P. aeruginosa SG81. A representative experiment of two independent experiments with standard deviations of the duplicates is shown. *** p < 0.001.