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Table 3 List of bacterial strains and plasmids used in this study

From: A Method for generating marker-less gene deletions in multidrug-resistant Acinetobacter baumannii

Strain or plasmid Relevant characteristics Reference or source
A. baumannii strains   
R2 Wild-type clinical MDR isolate TTSH6013 624325/06 Network for Antimicrobial Resistance Surveillance (Singapore)
DB Wild-type clinical MDR isolate DB15354/07 Network for Antimicrobial Resistance Surveillance (Singapore)
R2ΔadeFGH R2 with deletion in adeFGH operon This study
R2ΔadeIJK R2 with deletion in adeIJK operon This study
R2ΔadeFGH ΔadeIJK R2 with deletion in adeFGH and adeIJK operons This study
DBΔadeFGH DB with deletion in adeFGH operon This study
DBΔadeIJK DB with deletion in adeIJK operon This study
DBΔadeFGH ΔadeIJK DB with deletion in adeFGH and adeIJK operons This study
E. coli strains   
DH5α F– Φ80lac ZΔM15 Δ(lac ZYA-arg F) U169 rec A1 end A1 hsd R17 pho A sup E44 λ– thi-1 gyr A96 rel A1 Invitrogen
S17-1 Genotype: recA pro hsdR RP4-2-Tc::Mu-Km::Tn7, GmS [16]
Plasmids   
pMo130 Suicide plasmid, xylE+, sacB+, KmR [8]
pwFRT-TelR Donor of tellurite resistance cassette [10]
pMo130-TelR pMo130 plasmid containing 3.26 kb Xma I-digested tellurite-resistance cassette from pwFRT-TelR This study
pMo130-TelR-P8(UP/DWN) pMo130-TelR containing a 1 kb UP fragment (promoterless adeL) and 1 kb DOWN fragment (3’ partial adeH) This study
pMo130-TelR-adeJ(Up/Down) pMo130-TelR containing a 1 kb UP fragment (5’ partial adeI) and 0.9 kb DOWN fragment (3’ partial adeK) This study