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Table 3 List of bacterial strains and plasmids used in this study

From: A Method for generating marker-less gene deletions in multidrug-resistant Acinetobacter baumannii

Strain or plasmid

Relevant characteristics

Reference or source

A. baumannii strains

  

R2

Wild-type clinical MDR isolate TTSH6013 624325/06

Network for Antimicrobial Resistance Surveillance (Singapore)

DB

Wild-type clinical MDR isolate DB15354/07

Network for Antimicrobial Resistance Surveillance (Singapore)

R2ΔadeFGH

R2 with deletion in adeFGH operon

This study

R2ΔadeIJK

R2 with deletion in adeIJK operon

This study

R2ΔadeFGH ΔadeIJK

R2 with deletion in adeFGH and adeIJK operons

This study

DBΔadeFGH

DB with deletion in adeFGH operon

This study

DBΔadeIJK

DB with deletion in adeIJK operon

This study

DBΔadeFGH ΔadeIJK

DB with deletion in adeFGH and adeIJK operons

This study

E. coli strains

  

DH5α

F– Φ80lac ZΔM15 Δ(lac ZYA-arg F) U169 rec A1 end A1 hsd R17 pho A sup E44 λ– thi-1 gyr A96 rel A1

Invitrogen

S17-1

Genotype: recA pro hsdR RP4-2-Tc::Mu-Km::Tn7, GmS

[16]

Plasmids

  

pMo130

Suicide plasmid, xylE+, sacB+, KmR

[8]

pwFRT-TelR

Donor of tellurite resistance cassette

[10]

pMo130-TelR

pMo130 plasmid containing 3.26 kb Xma I-digested tellurite-resistance cassette from pwFRT-TelR

This study

pMo130-TelR-P8(UP/DWN)

pMo130-TelR containing a 1 kb UP fragment (promoterless adeL) and 1 kb DOWN fragment (3’ partial adeH)

This study

pMo130-TelR-adeJ(Up/Down)

pMo130-TelR containing a 1 kb UP fragment (5’ partial adeI) and 0.9 kb DOWN fragment (3’ partial adeK)

This study