From: A Method for generating marker-less gene deletions in multidrug-resistant Acinetobacter baumannii
Strain or plasmid | Relevant characteristics | Reference or source |
---|---|---|
A. baumannii strains | ||
R2 | Wild-type clinical MDR isolate TTSH6013 624325/06 | Network for Antimicrobial Resistance Surveillance (Singapore) |
DB | Wild-type clinical MDR isolate DB15354/07 | Network for Antimicrobial Resistance Surveillance (Singapore) |
R2ΔadeFGH | R2 with deletion in adeFGH operon | This study |
R2ΔadeIJK | R2 with deletion in adeIJK operon | This study |
R2ΔadeFGH ΔadeIJK | R2 with deletion in adeFGH and adeIJK operons | This study |
DBΔadeFGH | DB with deletion in adeFGH operon | This study |
DBΔadeIJK | DB with deletion in adeIJK operon | This study |
DBΔadeFGH ΔadeIJK | DB with deletion in adeFGH and adeIJK operons | This study |
E. coli strains | ||
DH5α | F– Φ80lac ZΔM15 Δ(lac ZYA-arg F) U169 rec A1 end A1 hsd R17 pho A sup E44 λ– thi-1 gyr A96 rel A1 | Invitrogen |
S17-1 | Genotype: recA pro hsdR RP4-2-Tc::Mu-Km::Tn7, GmS | [16] |
Plasmids | ||
pMo130 | Suicide plasmid, xylE+, sacB+, KmR | [8] |
pwFRT-TelR | Donor of tellurite resistance cassette | [10] |
pMo130-TelR | pMo130 plasmid containing 3.26 kb Xma I-digested tellurite-resistance cassette from pwFRT-TelR | This study |
pMo130-TelR-P8(UP/DWN) | pMo130-TelR containing a 1 kb UP fragment (promoterless adeL) and 1 kb DOWN fragment (3’ partial adeH) | This study |
pMo130-TelR-adeJ(Up/Down) | pMo130-TelR containing a 1 kb UP fragment (5’ partial adeI) and 0.9 kb DOWN fragment (3’ partial adeK) | This study |