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Table 2 Fold-change in fluorescence of H33342 at steady state level accumulation in the presence of EIs in efflux pump mutants and parental strains

From: A Method for generating marker-less gene deletions in multidrug-resistant Acinetobacter baumannii

Bacterial strain

+CCCPa

+PAβNb

DB

1.51 ± 0.04

1.29 ± 0.11

DBΔadeFGH

1.27 ± 0.12

1.28 ± 0.03

DBΔadeIJK

1.16 ± 0.06

1.24 ± 0.13

DBΔadeFGH ΔadeIJK

1.19 ± 0.03

1.36 ± 0.07

R2

1.31 ± 0.12

1.27 ± 0.04

R2ΔadeFGH

1.46 ± 0.04

1.29 ± 0.03

R2ΔadeIJK

1.09 ± 0.01

1.29 ± 0.05

R2ΔadeFGH ΔadeIJK

1.10 ± 0.01

1.20 ± 0.10

  1. Three separate experiments showed consistent results and representative examples are shown. The standard deviation represents variation between three biological replicates. All values shown are significant differences (P ≤ 0.05) in accumulation with addition of an EI relative to absence of EI.
  2. a fold-change compared to corresponding bacterial sample in the absence of CCCP.
  3. b fold-change compared to corresponding bacterial sample in the absence of PAβN.