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Figure 2 | BMC Microbiology

Figure 2

From: A Method for generating marker-less gene deletions in multidrug-resistant Acinetobacter baumannii

Figure 2

Verification of genomic deletions by PCR of genomic DNA. Amplimers using AdeGUp (NotI)F and AdeGDwn(SphI)R for DB (lane 1) and DBΔadeFGH (lane 3) DNA; amplimers using AdeG RTF and AdeG RTR for DB (lane 2) and DBΔadeFGH (lane 4) DNA; amplimers using AdeGUp (NotI)F and AdeGDwn(SphI)R for R2 (lane 5) and R2ΔadeFGH (lane 7) DNA; amplimers using AdeG RTF and AdeG RTR for R2 (lane 6) and R2ΔadeFGH (lane 8) DNA; amplimers using AdeJ(UP) PstI F and AdeJ(DWN) SphI R for R2 (lane 9), R2ΔadeIJK (lane 11), DB (lane 13) and DBΔadeIJK (lane 15); amplimers using AdeJ F and AdeK R for R2 (lane 10), R2ΔadeIJK (lane 12), DB (lane 14) and DBΔadeIJK (lane 16); DBΔadeFGH ΔadeIJK DNA amplified using AdeGUp (NotI)F and AdeGDwn(SphI)R (lane 17), AdeG RTF and AdeG RTR (lane 18); AdeJ(UP) PstI F and AdeJ(DWN) SphI R (lane 19) and AdeJ F and AdeK R (lane 20); R2ΔadeFGH ΔadeIJK DNA amplified with AdeGUp (NotI)F and AdeGDwn(SphI)R (lane 21), AdeG RTF and AdeG RTR (lane 22), AdeJ(UP) PstI F and AdeJ(DWN) SphI R (lane 23) and AdeJ F and AdeK R (lane 24); M, 1 kb DNA ladder (GeneRuler™).

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