Figure 3From: Monitoring the prevalence of viable and dead cariogenic bacteria in oral specimens and in vitro biofilms by qPCR combined with propidium monoazideEffect of oral specimens on qPCR. Samples of serially diluted S. sobrinus chromosomal DNA and S. sobrinus chromosomal DNA spiked with DNA from S. sobrinus-free oral specimens were analyzed by S. sobrinus-specific qPCR. Spike experiments with (A) saliva and (B) dental plaque. Saliva (+), S. sobrinus DNA with DNA from S. sobrinus-free saliva. Saliva (−), S. sobrinus DNA only. Plaque (+), S. sobrinus DNA with DNA from S. sobrinus-free dental plaque. Plaque (−), S. sobrinus DNA only. All experiments were performed independently three times. Means ± S.D. are shown.Back to article page