Protein level identification of the Listeria monocytogenes Sigma H, Sigma L, and Sigma C regulons

BMC Microbiology

Table 3 Proteins found to be differentially regulated by σ^C, as determined by a proteomic comparison between L. monocytogenes 10403S Δ BHL and Δ BCHL

Protein^a	Fold change ΔBHL/ΔBCHL	Description	Gene name	Role category^b	Sub-Role category^b
Proteins with positive fold change ( > 1.5) and p < 0.05 (indicating positive regulation by σ^C)
Lmo0096^c	3.19	mannose-specific PTS system IIAB component ManL	mptA	Energy metabolism	Pyruvate dehydrogenase
				Amino acid biosynthesis	Aromatic amino acid family
				Transport and binding proteins	Carbohydrates, organic alcohols, and acids
Proteins with negative fold change ( < -1.5) and p < 0.05 (indicating negative regulation by σ^C)
Lmo2094	−1.82	hypothetical protein	lmo2094	Energy metabolism	Sugars
Lmo1902	−1.61	3-methyl-2-oxobutanoate hydroxymethyltransferase	panB	Biosynthesis of cofactors, prosthetic groups, and carriers	Pantothenate and coenzyme A

^aProtein names are based on the L. monocytogenes EGD-e locus.
^bRole Categories and Sub-Role categories are based on JCVI classification [26].
^cPreceded by a putative σ^L promoter; tggca cagaacttgca; -12 and -24 regions are underlined.

ISSN: 1471-2180