Evidence for isolated evolution of deep-sea ciliate communities through geological separation and environmental selection

Table 4 Primer sets used in this study for the specific amplification of ciliate V4-SSU rRNA fragments using a two-step (nested) PCR reaction

			Primer	Primer sequences	Reference
1. Reaction: PCR ciliate specific primers
			Cil F	5′-TGGTAGTGTATTGGACWACCA-3′	[105]
			Cil R1	5′-TCTGATCGTCTTTGATCCCTT-3′	[105]
			Cil R2	5′-TCTRATCGTCTTTGATCCCCTA-3′	[105]
			Cil R3	5′-TCTGATTGTCTTTGATCCCCT-3′	[105]
2. Reaction: PCR hyper variable V4-region
Linker sequences	Key	MID	Primer	Primer sequences	Reference
5′-CGTATCGCCTCCCTCGCGCCA	TCAG	MID	TAReuk454FWD1	5′-CCAGCASCYGCGGTAATTCC-3′	[16]
5′-CGTATCGCCTCCCTCGCGCCA	TCAG	MID	TAReukREV3	5′-ACTTTCGTTCTTGATYRA-3′	[16]

ISSN: 1471-2180