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Table 3 Expected fragment sizes obtained with in silico digestion of the hsp60 gene sequences

From: Identification of species belonging to the Bifidobacterium genus by PCR-RFLP analysis of a hsp60 gene fragment

Bifidobacterium species

GenBank entry

Predicted fragment sizes

Profile

B. adolescentis

AF210319

31-36-81-103-339

 

B. angulatum

AF240568

42-54-59-139-296

 

B. animalis subsp. animalis

AY004273

17-53-86-97-114-223

 

B. animalis subsp. lactis

AY004282

71-86-96-114-223

 

B. asteroides

AF240570

30-38-75-97-109-242

 

B. bifidum

AY004280

22-31-59-181-297

 

B. boum

AY004285

22-117-200-251

 

B. breve

AF240566

106-139-139-200

 

B. catenulatum

AY004272

53-198-338

 

B. choerinum

AY013247

36-42-51-52-54-59-97-200

 

B. coryneforme

AY004275

16-32-54-158-338

 

B. cuniculi

AY004283

16-42-53-70-128-281

 

B. dentium

AF240572

22-31-42-68-130-139-158

 

B. gallicum

AF240575

42-253-297

 

B. gallinarum

AY004279

16-31-42-81-139-281

 

B. indicum

AF240574

16-32-36-42-45-123-296

 

B. longum subsp. longum

AF240578

42-113-138-139-158

*

B. longum subsp. infantis

AF240577

42-113-138-139-158

*

B. longum subsp. suis

AY013248

42-113-138-139-158

*

B. merycicum

AY004277

22-31-42-59-139-297

 

B. minimum

AY004284

16-51-60-66-70-327

 

B. pseudocatenulatum

AY004274

42-53-198-297

 

B. pseudolongum subsp pseudolongum

AY004282

17-22-30-32-42-42-109-297

 

B. pseudolongum subsp. globosum

AF286736

16-17-22-30-32-42-109-323

 

B. pullorum

AY004278

16-31-36-42-81-87-297

 

B. ruminantium

AF240571

31-106-114-339

 

B. subtile

Not available

Not avaiable

+

B. thermacidophilum subsp porcinum

AY004276

20-42-53-59-97-139-180

*†

B. thermacidophilum subsp thermacidophilum

AY004276

20-42-53-59-97-139-180

*†

B. thermophilum

AF240567

54-59-117-139-222

 
  1. + hsp60 sequence of B. subtile type strain was not available in the press-time.
  2. † the available sequences at GeneBank and cpnDB belonged to B. thermacidophilum (with no distinction in subspecies).
  3. *subspecies not discernable.