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Table 1 Quantification of Pseudomonas aeruginosa in CF sputum samples by culture and the opr L qPCR and detection by the gyr B/ ecf X qPCR

From: Proposal of a quantitative PCR-based protocol for an optimal Pseudomonas aeruginosa detection in patients with cystic fibrosis

CF patient CF sputum sample Quantification (CFU/mL) Multiplex qPCR detection (gyrB/ecfX)
Anonymisation number P. aeruginosacategory* By culture By oprL qPCR**
003 F 1 0.0E + 00 7.5E + 00 -/-
   2 0.0E + 00 1.4E + 03 +/-
   3 2.0E + 05 2.7E + 06 +/+
004 F 4 2.0E + 03 1.2E + 05 +/+
010 F 5 1.0E + 04 9.9E + 06 +/+
012 F 6 0.0E + 00 5.0E + 01 +/-
   7 0.0E + 00 7.5E + 01 -/-
   8 0.0E + 00 2.1E + 02 -/-
   9 1.0E + 07 7.8E + 06 +/-
013 F 10 1.0E + 08 4.0E + 09 +/+
014 N 11 1.0E + 06 5.5E + 06 +/+
023 N 12 4.0E + 01 2.5E + 03 +/-
024 F 13 1.0E + 03 1.3E + 05 +/+
025 N 14 5.0E + 04 4.3E + 07 +/+
   15 1.0E + 05 3.8E + 03 +/+
026 N 16 2.0E + 06 6.7E + 07 +/+
028 F 17 1.0E + 04 1.1E + 05 +/+
030 F 18 1.0E + 03 1.3E + 04 +/+
031 N 19 1.0E + 06 1.2E + 07 +/+
   20 2.0E + 07 1.0E + 08 +/+
034 F 21 4.0E + 02 6.8E + 04 +/+
035 F 22 1.0E + 04 2.7E + 04 +/+
040 F 23 1.0E + 06 1.4E + 06 +/+
041 F 24 1.0E + 02 4.9E + 01 +/-
043 N 25 6.0E + 02 5.6E + 06 +/+
047 N 26 0.0E + 00 1.1E + 03 +/+
   27 0.0E + 00 5.3E + 03 +/+
   28 1.0E + 07 1.1E + 07 +/+
048 F 29 0.0E + 00 8.1E + 02 +/+
   30 4.0E + 01 2.5E + 02 +/+
053 F 31 1.0E + 02 5.1E + 03 +/+
054 N 32 0.0E + 00 2.3E + 01 -/-
   33 2.0E + 05 3.7E + 06 +/+
057 F 34 1.0E + 06 2.0E + 01 -/-
060 F 35 4.0E + 06 1.5E + 08 +/+
061 F 36 1.0E + 02 6.1E + 03 +/+
066 F 37 4.0E + 03 3.1E + 04 +/+
   38 1.0E + 04 9.5E + 06 +/+
070 N 39 1.0E + 06 9.0E + 07 +/+
072 F 40 4.0E + 04 7.8E + 07 +/+
076 F 41 1.0E + 03 1.5E + 04 +/+
078 F 42 1.0E + 02 2.0E + 04 +/+
202 F 43 1.0E + 05 1.7E + 05 +/-
205 F 44 1.0E + 03 3.3E + 06 +/+
220 F 45 1.0E + 06 2.3E + 08 +/+
256 N 46 1.0E + 03 3.4E + 04 +/+
mean    3.3E + 06 1.2E + 08 NA
  1. *F: Free; N: Never (Lee et al., 2003).
  2. **mean of quantification by oprL qPCR tested in duplicate.
  3. NA: not applicable.
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BMC Microbiology

ISSN: 1471-2180

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