CF patient
|
CF sputum sample
|
Quantification (CFU/mL)
|
Multiplex qPCR detection (gyrB/ecfX)
|
---|
Anonymisation number
|
P. aeruginosacategory*
|
By culture
|
By oprL qPCR**
|
---|
003
|
F
|
1
|
0.0E + 00
|
7.5E + 00
|
-/-
|
| |
2
|
0.0E + 00
|
1.4E + 03
|
+/-
|
| |
3
|
2.0E + 05
|
2.7E + 06
|
+/+
|
004
|
F
|
4
|
2.0E + 03
|
1.2E + 05
|
+/+
|
010
|
F
|
5
|
1.0E + 04
|
9.9E + 06
|
+/+
|
012
|
F
|
6
|
0.0E + 00
|
5.0E + 01
|
+/-
|
| |
7
|
0.0E + 00
|
7.5E + 01
|
-/-
|
| |
8
|
0.0E + 00
|
2.1E + 02
|
-/-
|
| |
9
|
1.0E + 07
|
7.8E + 06
|
+/-
|
013
|
F
|
10
|
1.0E + 08
|
4.0E + 09
|
+/+
|
014
|
N
|
11
|
1.0E + 06
|
5.5E + 06
|
+/+
|
023
|
N
|
12
|
4.0E + 01
|
2.5E + 03
|
+/-
|
024
|
F
|
13
|
1.0E + 03
|
1.3E + 05
|
+/+
|
025
|
N
|
14
|
5.0E + 04
|
4.3E + 07
|
+/+
|
| |
15
|
1.0E + 05
|
3.8E + 03
|
+/+
|
026
|
N
|
16
|
2.0E + 06
|
6.7E + 07
|
+/+
|
028
|
F
|
17
|
1.0E + 04
|
1.1E + 05
|
+/+
|
030
|
F
|
18
|
1.0E + 03
|
1.3E + 04
|
+/+
|
031
|
N
|
19
|
1.0E + 06
|
1.2E + 07
|
+/+
|
| |
20
|
2.0E + 07
|
1.0E + 08
|
+/+
|
034
|
F
|
21
|
4.0E + 02
|
6.8E + 04
|
+/+
|
035
|
F
|
22
|
1.0E + 04
|
2.7E + 04
|
+/+
|
040
|
F
|
23
|
1.0E + 06
|
1.4E + 06
|
+/+
|
041
|
F
|
24
|
1.0E + 02
|
4.9E + 01
|
+/-
|
043
|
N
|
25
|
6.0E + 02
|
5.6E + 06
|
+/+
|
047
|
N
|
26
|
0.0E + 00
|
1.1E + 03
|
+/+
|
| |
27
|
0.0E + 00
|
5.3E + 03
|
+/+
|
| |
28
|
1.0E + 07
|
1.1E + 07
|
+/+
|
048
|
F
|
29
|
0.0E + 00
|
8.1E + 02
|
+/+
|
| |
30
|
4.0E + 01
|
2.5E + 02
|
+/+
|
053
|
F
|
31
|
1.0E + 02
|
5.1E + 03
|
+/+
|
054
|
N
|
32
|
0.0E + 00
|
2.3E + 01
|
-/-
|
| |
33
|
2.0E + 05
|
3.7E + 06
|
+/+
|
057
|
F
|
34
|
1.0E + 06
|
2.0E + 01
|
-/-
|
060
|
F
|
35
|
4.0E + 06
|
1.5E + 08
|
+/+
|
061
|
F
|
36
|
1.0E + 02
|
6.1E + 03
|
+/+
|
066
|
F
|
37
|
4.0E + 03
|
3.1E + 04
|
+/+
|
| |
38
|
1.0E + 04
|
9.5E + 06
|
+/+
|
070
|
N
|
39
|
1.0E + 06
|
9.0E + 07
|
+/+
|
072
|
F
|
40
|
4.0E + 04
|
7.8E + 07
|
+/+
|
076
|
F
|
41
|
1.0E + 03
|
1.5E + 04
|
+/+
|
078
|
F
|
42
|
1.0E + 02
|
2.0E + 04
|
+/+
|
202
|
F
|
43
|
1.0E + 05
|
1.7E + 05
|
+/-
|
205
|
F
|
44
|
1.0E + 03
|
3.3E + 06
|
+/+
|
220
|
F
|
45
|
1.0E + 06
|
2.3E + 08
|
+/+
|
256
|
N
|
46
|
1.0E + 03
|
3.4E + 04
|
+/+
|
mean
| | |
3.3E + 06
|
1.2E + 08
|
NA
|
- *F: Free; N: Never (Lee et al., 2003).
- **mean of quantification by oprL qPCR tested in duplicate.
- NA: not applicable.