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Table 1 Quantification of Pseudomonas aeruginosa in CF sputum samples by culture and the opr L qPCR and detection by the gyr B/ ecf X qPCR

From: Proposal of a quantitative PCR-based protocol for an optimal Pseudomonas aeruginosa detection in patients with cystic fibrosis

CF patient

CF sputum sample

Quantification (CFU/mL)

Multiplex qPCR detection (gyrB/ecfX)

Anonymisation number

P. aeruginosacategory*

By culture

By oprL qPCR**

003

F

1

0.0E + 00

7.5E + 00

-/-

  

2

0.0E + 00

1.4E + 03

+/-

  

3

2.0E + 05

2.7E + 06

+/+

004

F

4

2.0E + 03

1.2E + 05

+/+

010

F

5

1.0E + 04

9.9E + 06

+/+

012

F

6

0.0E + 00

5.0E + 01

+/-

  

7

0.0E + 00

7.5E + 01

-/-

  

8

0.0E + 00

2.1E + 02

-/-

  

9

1.0E + 07

7.8E + 06

+/-

013

F

10

1.0E + 08

4.0E + 09

+/+

014

N

11

1.0E + 06

5.5E + 06

+/+

023

N

12

4.0E + 01

2.5E + 03

+/-

024

F

13

1.0E + 03

1.3E + 05

+/+

025

N

14

5.0E + 04

4.3E + 07

+/+

  

15

1.0E + 05

3.8E + 03

+/+

026

N

16

2.0E + 06

6.7E + 07

+/+

028

F

17

1.0E + 04

1.1E + 05

+/+

030

F

18

1.0E + 03

1.3E + 04

+/+

031

N

19

1.0E + 06

1.2E + 07

+/+

  

20

2.0E + 07

1.0E + 08

+/+

034

F

21

4.0E + 02

6.8E + 04

+/+

035

F

22

1.0E + 04

2.7E + 04

+/+

040

F

23

1.0E + 06

1.4E + 06

+/+

041

F

24

1.0E + 02

4.9E + 01

+/-

043

N

25

6.0E + 02

5.6E + 06

+/+

047

N

26

0.0E + 00

1.1E + 03

+/+

  

27

0.0E + 00

5.3E + 03

+/+

  

28

1.0E + 07

1.1E + 07

+/+

048

F

29

0.0E + 00

8.1E + 02

+/+

  

30

4.0E + 01

2.5E + 02

+/+

053

F

31

1.0E + 02

5.1E + 03

+/+

054

N

32

0.0E + 00

2.3E + 01

-/-

  

33

2.0E + 05

3.7E + 06

+/+

057

F

34

1.0E + 06

2.0E + 01

-/-

060

F

35

4.0E + 06

1.5E + 08

+/+

061

F

36

1.0E + 02

6.1E + 03

+/+

066

F

37

4.0E + 03

3.1E + 04

+/+

  

38

1.0E + 04

9.5E + 06

+/+

070

N

39

1.0E + 06

9.0E + 07

+/+

072

F

40

4.0E + 04

7.8E + 07

+/+

076

F

41

1.0E + 03

1.5E + 04

+/+

078

F

42

1.0E + 02

2.0E + 04

+/+

202

F

43

1.0E + 05

1.7E + 05

+/-

205

F

44

1.0E + 03

3.3E + 06

+/+

220

F

45

1.0E + 06

2.3E + 08

+/+

256

N

46

1.0E + 03

3.4E + 04

+/+

mean

  

3.3E + 06

1.2E + 08

NA

  1. *F: Free; N: Never (Lee et al., 2003).
  2. **mean of quantification by oprL qPCR tested in duplicate.
  3. NA: not applicable.