Genomic and phenotypic characterization of in vitro-generated Chlamydia trachomatis recombinants

BMC Microbiology

Table 3 Analysis of independent recombinant strains for recombination hot-spots

Individual recombinant progeny from independent crosses were subjected to PCR-based DNA sequencing and assessed for recombination at positions identified as hotspots by Srinivasan and colleagues [24]. For each sequenced product, the identified genotype at that region is indicated (D or L1/L3). There were no examples of recombination in any of these sequenced PCR products.

ISSN: 1471-2180