BMC Microbiology

Table 1 Phenotypes of parents and progeny in recombinant crosses

From: Genomic and phenotypic characterization of in vitro-generated Chlamydia trachomatis recombinants

				MIC (μg/ml)				Phenotype
Cross	Progeny	Parental strains	OmpA	Rif	Ofl	Tet	Plasmid	Inclusion fusion	Attachment	2° inclusion formation
1	RC-J/6276tet		J	8	0.5	8	-	+	-	2
		L2-434tet-13	L2	0.008	16	8	+	+	+	1
		J/6276rif	J	8	0.5	0.032	-	+	-	1
2	RC-F(s)/342		F	32	0.5	8	-	-	-	N/A
		RC-J/6276tet-rif	J	8	0.5	8	-	+	-	1
		F(s)/70rif	F	32	0.5	0.032	-	-	-	N/A
3	RC-L2(s)/46		L2	32	16	0.032	+	-	+	N/A
		L2-434ofl	L2	0.008	16	0.032	+	+	+	1
		F(s)70rif	F	32	0.5	0.032	-	-	-	N/A
4	RC-F/69		F	32	4	0.032	+	+	+	1
		L2-434ofl	L2	0.008	16	0.032	+	+	+	1
		F(s)70rif	F	32	0.5	0.032	-	-	-	N/A
5	RC-L2(s)/3		L2	32	4	0.032	-	-	+	N/A
		L2-434ofl	L2	0.008	16	0.032	+	+	+	1
		F(s)70rif	F	32	0.5	0.032	-	-	-	N/A
6	RC-L2/55		L2	32	4	0.032	-	+	+	N/A
		L2-434ofl	L2	0.008	16	0.032	+	+	+	1
		F(s)/70rif	F	32	0.5	0.032	-	-	-	N/A
7	RC-J/953		J	8	16	0.032	+	+	+	4
		L2-434ofl	L2	0.008	16	0.032	+	+	+	1
		RC-F(s)/343tet-rif	F	32	0.5	8	-	-	-	N/A
		J/6276rifR	J	8	0.5	0.032	-	+	-	1
8	RC-J/943		J	8	16	0.032	+	+	+	1
		L2-434/ofl	L2	0.008	16	0.032	+	+	+	1
		RC-F(s)/343tet-rif	F	32	0.5	8	-	-	-	N/A
		J/6276rif	J	8	0.05	0.032	-	+	-	1
9	RC-J/966		J	8	16	0.032	+	+	+	1
		L2-434/ofl	L2	0.008	16	0.032	+	+	+	1
		RC-F(s)/343tet-rif	F	32	0.5	8	-	-	-	N/A
		J/6276rif	J	8	0.5	0.032	-	+	-	1
10	RC-L2/971		J	8	16	0.032	+	+	+	4
		L2-434/ofl	L2	0.008	16	0.032	+	+	+	1
		RC-F(s)/343tet-rif	F	32	0.5	8	-	-	-	N/A
		J/6276rif	J	8	0.5	0.032	-	+	-	1
11	RC-F(s)/852		F	32	4	8	+	-	-	N/A
		RC-F/69	F	32	4	0.032	+	+	+	1
		RC-F(s)/343tet-rif	F	32	0.5	8	-	-	-	N/A
12	RC-J(s)/122		J	32	4	8	-	-	+	N/A
		RC-L2(s)/3	L2	32	4	0.032	-	-	+	N/A
		RC-J/6276tet-rif	J	8	0.5	8	-	+	-	1

Plasmid phenotype tests for presence and genotype of plasmid.
Progeny strains used in subsequent recombinant crosses are shown in bold face.

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ISSN: 1471-2180

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