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Table 1 Strains and plasmids used in this study

From: Moraxella catarrhalis uses a twin-arginine translocation system to secrete the β-lactamase BRO-2

Strain Description Source
M. catarrhalis   
O35E WT isolate from middle ear effusion (Dallas, TX) [94]
O35E.TA tatA isogenic mutant of strain O35E, kanR This study
O35E.TB tatB isogenic mutant of strain O35E, kanR This study
O35E.TC tatC isogenic mutant of strain O35E, kanR This study
O35E.Bro bro-2 isogenic mutant of strain O35E, kanR This study
O12E WT isolate from middle ear effusion (Dallas, TX) [28]
O12E.TC tatC isogenic mutant of strain O12E, kanR This study
McGHS1 WT isolate from patient with respiratory infection (Toledo, OH) [33]
TTA37 WT isolate from transtracheal aspirate (Johnson City, TN) [28]
V1171 WT isolate from nasopharynx of a healthy child (Chapel Hill, NC) [28]
H. influenzae   
DB117 Host strain for cloning experiments with pWW115 [95, 96]
E. coli   
EPI300 Cloning strain Epicentre®
Illumina®
Plasmids   
pCC1 Cloning vector, camR Epicentre®
Illumina®
pCC1.3 pCC1-based plasmid containing kanR marker, camRkanR [31]
pRB.TatA.5 Contains 886-nt insert specifying O35E tatA in pCC1, camR This study
pRB.TatB.1 Contains 858-nt insert specifying O35E tatB in pCC1, camR This study
pRB.TatC.2 Contains 1,018-nt insert specifying O12E tatC in pCC1, camR This study
pRB.TatC:kan pRB.TatC.2 in which a kanR marker disrupts the tatC ORF, camR kanR This study
pRB.Tat.1 Contains 2,083-nt insert specifying O35E tatABC locus in pCC1, camR This study
pRB.TatA:kan pRB.Tat.1 in which a kanR marker disrupts the tatA ORF, camR kanR This study
pRB.TatB:kan pRB.Tat.1 in which a kanR marker disrupts the tatB ORF, camR kanR This study
pRN.Bro11 Contains 994-nt insert specifying O35E bro-2 in pCC1, camR This study
pRB.Bro:kan pRN.Bro11 in which a kanR marker disrupts the bro-2 ORF, camR kanR This study
pTS.BroKK.Ec pRN.Bro11 in which 2 arginines in the signal sequence of the bro-2 gene product were replaced with 2 lysines by site-directed mutagenesis, camR This study
pWW115 M. catarrhalis-H. influenzae shuttle cloning vector, spcR [95]
pRB.TatA pWW115 into which the tatA insert of pRB.TatA.5 was subcloned, spcR This study
pRB.TatB pWW115 into which the tatB insert of pRB.TatB.1 was subcloned, spcR This study
pRB.TatC pWW115 into which the tatB insert of pRB.TatC.2 was subcloned, spcR This study
pRB.TAT pWW115 into which the tatABC locus of pRB.Tat.1 was subcloned, spcR This study
pTS.Bro pWW115 into which the bro-2 insert of pRN.Bro11 was subcloned, spcR This study
pTS.BroKK pWW115 into which the bro-2 insert of pTS.BroKK.Ec was subcloned, spcR This study