Table 1 Strains and plasmids used in this study

M. catarrhalis

O35E

WT isolate from middle ear effusion (Dallas, TX)

[94]

O35E.TA

tatA isogenic mutant of strain O35E, kan^R

This study

O35E.TB

tatB isogenic mutant of strain O35E, kan^R

This study

O35E.TC

tatC isogenic mutant of strain O35E, kan^R

This study

O35E.Bro

bro-2 isogenic mutant of strain O35E, kan^R

This study

O12E

WT isolate from middle ear effusion (Dallas, TX)

[28]

O12E.TC

tatC isogenic mutant of strain O12E, kan^R

This study

McGHS1

WT isolate from patient with respiratory infection (Toledo, OH)

[33]

TTA37

WT isolate from transtracheal aspirate (Johnson City, TN)

[28]

V1171

WT isolate from nasopharynx of a healthy child (Chapel Hill, NC)

[28]

H. influenzae

DB117

Host strain for cloning experiments with pWW115

[95, 96]

E. coli

EPI300

Cloning strain

Epicentre®

Illumina®

Plasmids

pCC1

Cloning vector, cam^R

Epicentre®

Illumina®

pCC1.3

pCC1-based plasmid containing kan^R marker, cam^Rkan^R

[31]

pRB.TatA.5

Contains 886-nt insert specifying O35E tatA in pCC1, cam^R

This study

pRB.TatB.1

Contains 858-nt insert specifying O35E tatB in pCC1, cam^R

This study

pRB.TatC.2

Contains 1,018-nt insert specifying O12E tatC in pCC1, cam^R

This study

pRB.TatC:kan

pRB.TatC.2 in which a kan^R marker disrupts the tatC ORF, cam^R kan^R

This study

pRB.Tat.1

Contains 2,083-nt insert specifying O35E tatABC locus in pCC1, cam^R

This study

pRB.TatA:kan

pRB.Tat.1 in which a kan^R marker disrupts the tatA ORF, cam^R kan^R

This study

pRB.TatB:kan

pRB.Tat.1 in which a kan^R marker disrupts the tatB ORF, cam^R kan^R

This study

pRN.Bro11

Contains 994-nt insert specifying O35E bro-2 in pCC1, cam^R

This study

pRB.Bro:kan

pRN.Bro11 in which a kan^R marker disrupts the bro-2 ORF, cam^R kan^R

This study

pTS.BroKK.Ec

pRN.Bro11 in which 2 arginines in the signal sequence of the bro-2 gene product were replaced with 2 lysines by site-directed mutagenesis, cam^R

This study

pWW115

M. catarrhalis-H. influenzae shuttle cloning vector, spc^R

[95]

pRB.TatA

pWW115 into which the tatA insert of pRB.TatA.5 was subcloned, spc^R

This study

pRB.TatB

pWW115 into which the tatB insert of pRB.TatB.1 was subcloned, spc^R

This study

pRB.TatC

pWW115 into which the tatB insert of pRB.TatC.2 was subcloned, spc^R

This study

pRB.TAT

pWW115 into which the tatABC locus of pRB.Tat.1 was subcloned, spc^R

This study

pTS.Bro

pWW115 into which the bro-2 insert of pRN.Bro11 was subcloned, spc^R

This study

pTS.BroKK

pWW115 into which the bro-2 insert of pTS.BroKK.Ec was subcloned, spc^R

This study