Table 2 Specificity-conferring amino acids and homologies of the adenylation domains in polymyxin synthetases of strains M-1, E681, and PKB1

Module 1

pmxE1/M-1

D

V

G

E

I

S

S

I

L-Dab

99

99

pmxE1/E681

D

V

G

E

I

S

S

I

L-Dab

pmxE1/PKB1

D

V

W

E

I

S

S

I

L-Dab

Module 2

pmxE2/M-1

D

F

W

N

I

G

M

V

L-Thr

99

98

pmxE2/E681

D

F

W

N

I

G

M

V

L-Thr

pmxE2/PKB1

D

F

W

N

I

G

M

V

L-Thr

Module 3 (W)

pmxE3/M-1

D

V

G

E

I

S

S

I

D-Dab

98

92

pmxE3/E681

D

V

G

E

I

S

S

I

D-Dab

pmxE3/PKB1

D

V

G

E

I

S

S

I

D-Dab

Module 4

pmxE4/M-1

D

V

G

E

I

S

A

I

L-Dab

96

96

pmxE4/E681

D

V

G

E

I

S

A

I

L-Dab

pmxE4/PKB1

D

V

G

E

I

S

A

I

L-Dab

Module 5

pmxE1/M-1

D

V

G

E

I

S

A

I

L-Dab

97

89

pmxE1/E681

D

V

G

E

I

S

A

I

L-Dab

pmxE1/PKB1

D

V

G

E

I

S

A

I

L-Dab

Module 6 (X)

pmxA1/M-1

D

A

W

T

I

A

A

I

D-Phe

88

99

pmxA1/E681

D

A

W

I

V

G

A

I

D-Leu

pmxA1/PKB1

D

A

W

T

I

A

A

I

D-Phe

Module 7 (Y)

pmxA2/M-1

D

F

W

N

I

G

M

V

L-Thr

99

51

pmxA2/E681

D

F

W

N

I

G

M

V

L-Thr

pmxA2/PKB1

D

G

F

L

L

G

L

V

L-Leu

Module 8

pmxA3/M-1

D

V

G

E

I

S

A

I

L-Dab

97

92

pmxA3/E681

D

V

G

E

I

S

A

I

L-Dab

pmxA3/PKB1

D

V

G

E

I

S

A

I

L-Dab

Module 9

pmxA4/M-1

D

V

G

E

I

S

A

I

L-Dab

96

91

pmxA4/E681

D

V

G

E

I

S

A

I

L-Dab

pmxA4/PKB1

D

V

G

E

I

S

A

I

L-Dab

Module 10 (Z)

pmxB1/M-1

D

F

W

N

I

G

M

V

L-Thr

97

99

pmxB1/E681

D

F

W

N

I

G

M

V

L-Thr

pmxB1/PKB1

D

F

W

N

I

G

M

V

L-Thr