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Table 2 Specificity-conferring amino acids and homologies of the adenylation domains in polymyxin synthetases of strains M-1, E681, and PKB1

From: Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1

Module/ strain Active site residues in A-domain Specified aa % aa E681 % aa PKB1
235 236 239 278 299 301 322 330
Module 1  
pmxE1/M-1 D V G E I S S I L-Dab 99 99
pmxE1/E681 D V G E I S S I L-Dab   
pmxE1/PKB1 D V W E I S S I L-Dab   
Module 2  
pmxE2/M-1 D F W N I G M V L-Thr 99 98
pmxE2/E681 D F W N I G M V L-Thr   
pmxE2/PKB1 D F W N I G M V L-Thr   
Module 3 (W)  
pmxE3/M-1 D V G E I S S I D-Dab 98 92
pmxE3/E681 D V G E I S S I D-Dab   
pmxE3/PKB1 D V G E I S S I D-Dab   
Module 4  
pmxE4/M-1 D V G E I S A I L-Dab 96 96
pmxE4/E681 D V G E I S A I L-Dab   
pmxE4/PKB1 D V G E I S A I L-Dab   
Module 5  
pmxE1/M-1 D V G E I S A I L-Dab 97 89
pmxE1/E681 D V G E I S A I L-Dab   
pmxE1/PKB1 D V G E I S A I L-Dab   
Module 6 (X)  
pmxA1/M-1 D A W T I A A I D-Phe 88 99
pmxA1/E681 D A W I V G A I D-Leu   
pmxA1/PKB1 D A W T I A A I D-Phe   
Module 7 (Y)  
pmxA2/M-1 D F W N I G M V L-Thr 99 51
pmxA2/E681 D F W N I G M V L-Thr   
pmxA2/PKB1 D G F L L G L V L-Leu   
Module 8  
pmxA3/M-1 D V G E I S A I L-Dab 97 92
pmxA3/E681 D V G E I S A I L-Dab   
pmxA3/PKB1 D V G E I S A I L-Dab   
Module 9  
pmxA4/M-1 D V G E I S A I L-Dab 96 91
pmxA4/E681 D V G E I S A I L-Dab   
pmxA4/PKB1 D V G E I S A I L-Dab   
Module 10 (Z)  
pmxB1/M-1 D F W N I G M V L-Thr 97 99
pmxB1/E681 D F W N I G M V L-Thr   
pmxB1/PKB1 D F W N I G M V L-Thr   
  1. Modules 3 and 6 contained extra epimerization domains which might convert Dab3 and Phe6 to the D-configuration. A-domains in modules 6 (X) and 7(Y) specifying different amino acids in the polymyxins produced by M-1, E681, and PKB1 are underlined.