Community acquisition of β-lactamase producing Enterobacteriaceae in neonatal gut

BMC Microbiology

Table 1 Primers used for detection of TEM, SHV and Carbapenemase genes

Primers	Primer Sequence (5′ to 3′ direction)	Annealing	Amplicon size
		Temperature (°C)	(bp)
TEM	FP- ATG AGT ATT CAA CAT TTC CG	50	858
	RP- CCA ATG CTT AAT CAG TGA GG
SHV	FP- ATG CGT TAT ATT CGC CTG TG	58	862
	RP- AGC GTT GCC AGT GCT CGA TC
KPC-1	FP- AGC CGT TAC AGC CTC TGG AG	55	1351
	RP- GAT GGG ATT GCG TCA GTT CAG
KPC-2	FP- CAC TGT ATC GCC GTC TAG TTC	55	812
	RP- TGT GCT TGT CAT CCT TGT TAG
NDM-1	FP- CGACGATTGGCCAGCAAATG	58	551
	RP- ACTTGGCCTTGCTGTCCTTG
IMP	FP- TTGAAAAGCTTGATGAAGGCG	58	616
	RP- ACCGCCTGCTCTAATGTAAG
VIM	FP- TTGACCGCGTCTATCATGGC	58	762

ISSN: 1471-2180