Table 6 Primers used in this study
From: Adaptive mechanisms of Campylobacter jejunito erythromycin treatment
Primers | Sequence*(5’ → 3’) (restriction enzyme) |
|---|---|
Primers for mutant construction | |
cj0308c-F(AF) | CTCGAATTCCCTTTTCCATAGATGTTTGC (EcoRI) |
cj0309c-R (AR) | CCAGGTACCAAATTTCACAGGCAGTAGCT(KpnI) |
cj0310c-F (BF) | GAATCTAGAGTGGGGTAATGATAGGAGTG(XbaI) |
cj0311-R (BR) | CCAGTCGACCAAGGGCTAAAAGATTGATA (SalI) |
cj0422c-F (CF) | GCCGAATTCAATAAGGGTTGTAATTGAAGCG (EcoRI) |
cj0423-R (CR) | AATGGTACCTGCTCAAACCAATAATAGCG(KpnI) |
cj0425-F (DF) | TTTGCTAGCGGACTA TTATTGTTTGGCTTGT (NheI) |
cj0426-R (DR) | ATCCTGCAGGTCTAATGAGTTGGCCTGAA (PstI) |
cj1168c-F(EF) | AGCGAATTCACAGAACCTAAAGTCCCA (EcoRI) |
cj1169c-R (ER) | AATCGGTACCATCCAGAGCCAGAGTCAAA(KpnI) |
cj1170c-F (FF) | AAATCTAGAATGAAAGAAGACAAGCACT(XbaI) |
cj1171c-R (FR) | TTGGCATGCTAGAAACTGAAAAAGGCAC (SphI) |
cj1172c-F (GF) | ACAGAATTCACGGTGCGTGTAGGGTT (EcoRI) |
cj1173-R (GR) | ACTGGTACCACAATAGCAGCAATCAAGAA(KpnI) |
cj1174-F (HF) | GGTTCTAGAGGCATCATAGGAACTTGTCT(XbaI) |
cj1175c-R (HR) | GGTCTGCAGATCGATGATGTAATGAAAGC (PstI) |
16S-F | ATCGTAGATCAGCCATGCTA |
Primers for complementation construction | |
cj1169c-cj1170c-F | TTGTCTAGAAAAAGATTAAACAGTAA(XbaI) |
cj1169c-cj1170c-R | AACTCTAGAACAAGGAGTTATGATTA(XbaI) |
cj1173-cj1174-F | TATTCTAGACAGTGCCACCTTCTTTAGCG(XbaI) |
cj1173-cj1174-R | CCGTCTAGACTTTAATGGGTATTGAAGCA(XbaI) |
Primers for qRT-PCR | |
qRT-16S-F | TCCCAGTTCGGATTGTTCTC |
qRT-16S-R | GTACAAGACCCGGGAACGTA |
cj0061-F | GAAGGCAAGCGTTCTTTTAGTCT |
cj0061-R | AGAAAGAGCAAGATGAGCTTGTG |
cj0258-F | GTGAAAATGCCGATGAAAATG |
cj0258-R | GCGATGATGGTAGGTTTACTTTG |
cj0310c-F | TAGAGTGCTTTTGGGTAAGTGGA |
cj0310c-R | AATACAAACCCCAATGGCTGTTA |
cj0345-F | TATGGTGTTGTTTTGGGTTCTTC |
cj0345-R | CCAGCAATAGGGGCTAAATAAAT |
cj0425-F | CTTCAGGCCAACTCATTAGA |
cj0425-R | GGGCTTTAAGTCCGGATAATTC |
cj0426-F | AACCTTGATTTAGAGGCGATTTC |
cj0426-R | GCCTATCATGAGAGATGACAACC |
cj0767-F | ATGGTTTCCATATCTTCCCAAAG |
cj0767-R | TAATCCGTGGACTTAGAGCAGTG |
cj1168c-F | CAAGTATAGCCACCCAAATAGCA |
cj1168c-R | TTACCAGGGATTCGTCAGTACAT |
cj1170-F | CAAAATCAGTCATATGAGCCACA |
cj1170-R | CTATGCTGCAGTAGAAGGAGAGC |
cj1173-F | CTGTATGGGAGCTTTTAGGGATT |
cj1173-R | TCCAACGATAGAAAGAACAATGC |
cj1226-F | GCTTTGCTTCTGTTATCTCATCC |
cj1226-R | AGGCTCTTAAATTTTGCAGGAGT |
cj1563-F | GCAGTTTTTATACCCAAAGAGCA |
cj1563-R | GCGATATAGAATGGGTAAAATGG |
Primers for conventional RT-PCR | |
cj0423-F | GCGGGTCTATTTTTGTA |
cj0425-R | CTTGGCTATTTCCTTGA |