Table 4 Primers used in this study
Name | Description | Application |
|---|---|---|
gspKO-up | GACAATCTTTTAATACAGACAAAGAGCATCTGCGAAAAATTGTACGCGGGATTCCGGGGATCCGTCGACC | gspC-M deletion |
gspKO-dn | CGCCACGTTAACGAGAGTAATTTTATTGATACTAATCTCCTGATACTTTATGTAGGCTGGAGCTGCTTCG | gspC-M deletion |
pppAKO-dn | ATACACTTGCAGGCCCGCATCCGGCAAGTTACAACAAACAACCTTTAACCATTCCGGGGATCCGTCGACC | pppA deletion |
pppAKO-dn | TTATTAATAAGAGTTAAAATGTCACTTTGATAATGACGTTGTTATCATTATGTAGGCTGGAGCTGCTTCG | pppA deletion |
sslEKO-up | TTTCTCCCAGTTACGAATTTTTTAACATTGTTTTGTCACTTGCGTTATTAATTCCGGGGATCCGTCGACC | sslE deletion |
sslEKO-dn | TTATTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATACAGGATTATGTAGGCTGGAGCTGCTTCG | sslE deletion |
pppA-up | TTATTAGGTACCATGCTTTTTGATGTTTTTCAGC | pppA cloning |
pppA-dn | ATATTAGGATCCTTAAAACAATGCCTGTAGATAAATTG | pppA cloning |
sslE-up | TTATTAGGTACCATGAATAAGAAATTTAAATATAAGAAATCG | sslE cloning |
sslE-noSP-up | TTATTAGGTACCATGTCTTCCTCCGATACG | sslE-SP cloning |
sslE-dn | TTATTAGGATCCTTACTCGACAGACATCTTATG | sslE cloning |
sslE-dn-nostop | TTATTAGGATCCGCTCTCGACAGACATCTTATG | sslE cloning for fusions |
cel45A-noSP-up | TTATTAGGATCCGCAGTTTGTGAATATCGTGTTACC | sslE-cel45A fusion |
cel45A-dn | TTATTAAAGCTTTTACGGGCAGGTATTACGAATATC | sslE-cel45A fusion |
pel10A-noSP-up | TTATTAGGATCCGCCTGCAGTTACAAGGTCAC | sslE-pel10A fusion |
pel10A-dn | TTATTAAAGCTTTTACAGGTAACCCACTTTCTGG | sslE-pel10A fusion |