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Table 4 Primers used in this study

From: The non-pathogenic Escherichia coli strain W secretes SslE via the virulence-associated type II secretion system beta

Name Description Application
gspKO-up GACAATCTTTTAATACAGACAAAGAGCATCTGCGAAAAATTGTACGCGGGATTCCGGGGATCCGTCGACC gspC-M deletion
gspKO-dn CGCCACGTTAACGAGAGTAATTTTATTGATACTAATCTCCTGATACTTTATGTAGGCTGGAGCTGCTTCG gspC-M deletion
pppAKO-dn ATACACTTGCAGGCCCGCATCCGGCAAGTTACAACAAACAACCTTTAACCATTCCGGGGATCCGTCGACC pppA deletion
pppAKO-dn TTATTAATAAGAGTTAAAATGTCACTTTGATAATGACGTTGTTATCATTATGTAGGCTGGAGCTGCTTCG pppA deletion
sslEKO-up TTTCTCCCAGTTACGAATTTTTTAACATTGTTTTGTCACTTGCGTTATTAATTCCGGGGATCCGTCGACC sslE deletion
sslEKO-dn TTATTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATACAGGATTATGTAGGCTGGAGCTGCTTCG sslE deletion
pppA-up TTATTAGGTACCATGCTTTTTGATGTTTTTCAGC pppA cloning
pppA-dn ATATTAGGATCCTTAAAACAATGCCTGTAGATAAATTG pppA cloning
sslE-up TTATTAGGTACCATGAATAAGAAATTTAAATATAAGAAATCG sslE cloning
sslE-noSP-up TTATTAGGTACCATGTCTTCCTCCGATACG sslE-SP cloning
sslE-dn TTATTAGGATCCTTACTCGACAGACATCTTATG sslE cloning
sslE-dn-nostop TTATTAGGATCCGCTCTCGACAGACATCTTATG sslE cloning for fusions
cel45A-noSP-up TTATTAGGATCCGCAGTTTGTGAATATCGTGTTACC sslE-cel45A fusion
cel45A-dn TTATTAAAGCTTTTACGGGCAGGTATTACGAATATC sslE-cel45A fusion
pel10A-noSP-up TTATTAGGATCCGCCTGCAGTTACAAGGTCAC sslE-pel10A fusion
pel10A-dn TTATTAAAGCTTTTACAGGTAACCCACTTTCTGG sslE-pel10A fusion