Strains
| | |
W
|
Wild-type E. coli W
|
ATCC 9637
|
W Δgsp::Kan
|
W ΔgspC-M::FRT-kan-FRT
|
This work
|
W Δgsp::FRT
|
W ΔgspC-M::FRT, derived by FLP recombination from W Δgsp::Kan
|
This work
|
W ΔpppA::Kan
|
W ΔpppA::FRT-kan-FRT
|
This work
|
W ΔpppA::FRT
|
W ΔpppA::FRT, derived by FLP recombination from W ΔpppA::Kan
|
This work
|
W ΔsslE::Kan
|
W ΔsslE::FRT-kan-FRT
|
This work
|
W ΔsslE::FRT
|
W ΔsslE::FRT, derived by FLP recombination from W ΔsslE::Kan
|
This work
|
Plasmids
| |
This work
|
pRH21
|
pACYC184-derived; trc promoter; lacIq
|
This work
|
pRH31
|
pTrc99A-derived; trc promoter; lacIq
|
This work
|
pMSD6
|
pRH21 with sslE cloned into the MCS
|
This work
|
pMSD7
|
pRH21 with sslE lacking the signal peptide-encoding sequence cloned into the MCS
|
This work
|
pMSD8
|
pRH21 with pppA cloned into the MCS
|
This work
|
pRH153
|
pRH31 with an sslE-cel45A fusion cloned into the MCS
|
This work
|
pRH154
|
pRH31 with an sslE-pel10A fusion cloned into the MCS
|
This work
|