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Table 3 Strains and plasmids used in this study

From: The non-pathogenic Escherichia coli strain W secretes SslE via the virulence-associated type II secretion system beta

E. colistrain or plasmid Descriptiona Reference or sourceb
Strains   
  W Wild-type E. coli W ATCC 9637
  W Δgsp::Kan W ΔgspC-M::FRT-kan-FRT This work
  W Δgsp::FRT W ΔgspC-M::FRT, derived by FLP recombination from W Δgsp::Kan This work
  W ΔpppA::Kan W ΔpppA::FRT-kan-FRT This work
  W ΔpppA::FRT W ΔpppA::FRT, derived by FLP recombination from W ΔpppA::Kan This work
  W ΔsslE::Kan W ΔsslE::FRT-kan-FRT This work
  W ΔsslE::FRT W ΔsslE::FRT, derived by FLP recombination from W ΔsslE::Kan This work
Plasmids   This work
  pRH21 pACYC184-derived; trc promoter; lacIq This work
  pRH31 pTrc99A-derived; trc promoter; lacIq This work
  pMSD6 pRH21 with sslE cloned into the MCS This work
  pMSD7 pRH21 with sslE lacking the signal peptide-encoding sequence cloned into the MCS This work
  pMSD8 pRH21 with pppA cloned into the MCS This work
  pRH153 pRH31 with an sslE-cel45A fusion cloned into the MCS This work
  pRH154 pRH31 with an sslE-pel10A fusion cloned into the MCS This work
  1. a MCS, multiple cloning site.
  2. b ATCC, American Type Culture Collection.
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BMC Microbiology

ISSN: 1471-2180

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