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Table 1 Extracellular and surface-displayed activity of SslE-Cel45A and SslE-Pel10A from liquid cultures

From: The non-pathogenic Escherichia coli strain W secretes SslE via the virulence-associated type II secretion system beta

  Strain SslE-Cel45A activitya SslE-Pel10A activitya
  Supernatant fractionb Displayed fractionc Supernatant fractionb Displayed fractionc
WT pRH153 < 0.01 < 0.01 -- --
ΔpppA pRH153 < 0.01 < 0.01 -- --
WT pRH154 -- -- 0.11(1) 0.08(1)
ΔpppA pRH154 -- -- 0.10(4) 0.095(5)
  1. a Values shown are means of at least two biological replicates, with error in the last digit denoted parenthetically.
  2. b Extracellular activity divided by the activity from an equivalent fraction of lysed culture.
  3. c Activity measured using intact cells divided by the activity from an equivalent fraction of lysed culture.
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BMC Microbiology

ISSN: 1471-2180

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