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Table 1 Extracellular and surface-displayed activity of SslE-Cel45A and SslE-Pel10A from liquid cultures

From: The non-pathogenic Escherichia coli strain W secretes SslE via the virulence-associated type II secretion system beta

  Strain

SslE-Cel45A activitya

SslE-Pel10A activitya

 

Supernatant fractionb

Displayed fractionc

Supernatant fractionb

Displayed fractionc

WT pRH153

< 0.01

< 0.01

--

--

ΔpppA pRH153

< 0.01

< 0.01

--

--

WT pRH154

--

--

0.11(1)

0.08(1)

ΔpppA pRH154

--

--

0.10(4)

0.095(5)

  1. a Values shown are means of at least two biological replicates, with error in the last digit denoted parenthetically.
  2. b Extracellular activity divided by the activity from an equivalent fraction of lysed culture.
  3. c Activity measured using intact cells divided by the activity from an equivalent fraction of lysed culture.