A unique midgut-associated bacterial community hosted by the cave beetle Cansiliella servadeii(Coleoptera: Leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors

Paoletti, Maurizio G; Mazzon, Luca; Martinez-Sañudo, Isabel; Simonato, Mauro; Beggio, Mattia; Dreon, Angelo Leandro; Pamio, Alberto; Brilli, Mauro; Dorigo, Luca; Summers Engel, Annette; Tondello, Alessandra; Baldan, Barbara; Concheri, Giuseppe; Squartini, Andrea

doi:10.1186/1471-2180-13-129

Table 2 Taxonomical assignment of cloned 16S rRNA amplicons from the midgut content of Cansiliella servadeii

From: A unique midgut-associated bacterial community hosted by the cave beetle Cansiliella servadeii(Coleoptera: Leptodirini) reveals parallel phylogenetic divergences from universal gut-specific ancestors

OTU #	Taxonomy	Clone and GenBank Code	Top database similarities (%)¹	Habitat²
1	Firmicutes	7B, (JQ308118)	(92.4) Unc³. bacterium AB606297	Mouse faeces
		7B, (JQ308118)	(92.1) Unc. Clostridiaceae AB088980	Reticulitermes speratus gut (Isoptera: Termitidae)
		43A;14B; 9B; 33C, (JQ308112, JQ308119, JQ308111, JQ308113)	(92.6) Unc. bacterium AB606297	Mouse faeces
			(92.4) Unc. bacterium DQ815954	Mouse cecum
			(92.3) Unc. Clostridiaceae AB088980	R. speratus gut
		19B; 23C; 25C; 28C; 39C, 50B, 53B, 57B, 73A, 74A (JQ308115, JQ308116, JQ308110, JQ308114, JQ308117, JX463078, JX463086, JX463088, JX463089), JX463090	(92.9) Unc. bacterium AB606297	Mouse faeces
			(92.6) Unc. Clostridiaceae AB088980	R. speratus gut
		41A, (JQ308120)	(93.1) Unc. bacterium AB606297	Mouse faeces
			(92.9) Unc. bacterium DQ815954	Mouse cecum
			(92.8) Unc. Clostridiaceae AB088980	R. speratus gut
		49B (JX463074)	(92.9) Unc. bacterium AB606297	Mouse faeces
			(92.6) Unc. bacterium DQ815954	Mouse cecum
			(92.5) Unc. Clostridiaceae AB088980	R. speratus gut
2	Firmicutes	10B, (JQ308121)	(92.3) Unc. bacterium EF602946	Mouse cecum
3	Firmicutes	4A; 42A, (JQ308123, JQ308124)	(95.9) Unc. Clostridiales AB088981	R. speratus gut
		4A; 42A, (JQ308123, JQ308124)	(94.4) Unc. bacterium GU451010	Tipula abdominalis gut (Diptera: Tipulidae)
		67A, 72A (JX463084, JX463085)	(94.8) Unc. Clostridiales AB088981	R. speratus gut
		8B, (JQ308122)	(95.5) Unc. bacteriumEF608549	Poecilus chalcites gut (Coleoptera: Carabidae)
4	Firmicutes	32C, (JQ308126)	(95.2) Unc. Clostridiaceae AB192046	Microcerotermes spp. gut (Isoptera: Termitidae)
4	Firmicutes	48A, 68A, 75A (JQ308127, JX463080, JX463091)	(95.7) Unc. bacterium AJ852374	Melolontha melolontha gut (Coleoptera: Scarabaeidae)
5	Firmicutes	21C, (JQ308125)	(94,5) Unc. bacterium FJ374218	Pachnoda spp. gut (Coleoptera: Scarabaeidae)
6	Firmicutes	2A;12B, (JQ308128, JQ308129)	(97.1) Unc. Clostridiaceae AB192046	Microcerotermes spp. gut (Isoptera: Termitidae)
		6B, (JQ308130)	(96.9) Unc. bacterium FJ374218	Pachnoda spp. larval gut (Coleoptera: Scarabaeidae)
		46A, 63A (JQ308131, JX463079)	(94.5) Unc. bacterium FJ374218	Pachnoda spp. gut (Coleoptera: Scarabaeidae)
7	Firmicutes	15B, (JQ308133)	(91.7) Unc. bacterium EU465991	African elephant faeces
		15B, (JQ308133)	(90.5) Unc. bacterium AY654956	Chicken gut
		29C, (JQ308132)	(91.9) Unc. bacterium EU465991	African elephant faeces
		29C, (JQ308132)	(90.7) Unc. bacterium AY654956	Chicken gut
8	Firmicutes	5A, (JQ308134)	(93.8) Unc. Clostridiales AB231035	Hodotermopsis sjoestedti gut (Isoptera: Termitidae)
9	Firmicutes	69A (JX463081)	(94.7) Unc. bacterium AB088973	R. speratus gut
10	Firmicutes	71A(JX463087)	(92.7) Unc. bacterium AB088973	R. speratus gut
11	Firmicutes	24C, 30C, (JQ308135, JQ308136)	(92.6) Unc. Firmicutes GQ275112	Leptogenys spp. gut (Hymenoptera: Formicidae)
12	Actinobacteria	61A (JX463076)	(93.2) Unc. Bacterium FR687129	Paddy soil
13	Actinobacteria	22C; 36C, 51B, 54B (JQ308137, JQ308138, JX463075, JX463083)	(97.2) Unc. bacterium DQ521505	Lake Vida ice cover
		22C; 36C, 51B, 54B (JQ308137, JQ308138, JX463075, JX463083)	(96.9) Unc. bacterium AM940404	Rhagium inquisitor gut (Coleoptera: Cerambycidae)
		52B (JX463077)	(96.7) Unc. bacterium DQ521505	Lake Vida, ice cover
		52B (JX463077)	(96.5) Unc. bacterium AM940404	Rhagium inquisitor gut (Coleoptera: Cerambycidae)
		65A (JX463082)	(97) Unc. bacterium DQ521505	Lake Vida, ice cover
		65A (JX463082)	(96.7) Unc. bacterium AM940404	Rhagium inquisitor gut (Coleoptera: Cerambycidae
14	Actinobacteria	45A, (JQ308139)	(99.5) Sanguibacter inulinus HQ326836	Thorectes lusitanicus gut (Coleoptera: Geotrupidae)
15	α-Proteobacteria	13B, (JQ308142)	(96.2) Unc. α-proteobacterium CU920098	Mesophilic anaerobic digester treating wastewater sludge
15	α-Proteobacteria		(93.7) Unc. bacterium FN659093	Lumbricus terrestris gut
16	α-Proteobacteria	58B (JX463098)	(100) Brevundimonas sp.JQ316297	Soil
17	α-Proteobacteria	44A (JQ308143)	(92.5) Unc. bacterium EF667926	Epithelium Hydra vulgaris
			(88.2) Unc. bacterium HM779996	Adult zebrafish gut
			(87.9) Unc. bacterium EU148629	Agrilus planipennis gut (Coleoptera: Buprestidae)
18	δ-Proteobacteria	3A; 20A, 62A (JQ308144, JQ308145, JX463096)	(94.3) Unc. δ-proteobacterium DQ307712	Macrotermes michaelsenigut (Isoptera: Termitidae)
19	δ-Proteobacteria	60B (JX463100)	(96) Unc. Desulfovibrionaceae JN653048	Gut of millipede Tachypodoiulus niger
20	δ-Proteobacteria	66A, 70A (JX463092, JX463093)	(94.1) Unc. bacterium FJ374259	P. ephippiata gut (Coleoptera: Scarabaeidae)
21	β-Proteobacteria	27C, (JQ308141)	(95.2) Unc.bacterium AJ852369	Melolontha melolontha gut (Coleoptera: Scarabaeidae)
22	β-Proteobacteria	26C, (JQ308140)	(96.5) Burkholderiales bacterium EU073950	Dermolepida albohirtum gut (Coleoptera: Scarabaeidae)
23	Bacteroidetes	11B, (JQ308146)	(91.9) Unc. bacterium AJ576327	Pachnoda ephippiata gut (Coleoptera: Scarabaeidae)
		18B, (JQ308147)	(92.1) Unc. bacterium HQ728219	Microbial fuel cell
		18B, (JQ308147)	(91.9) Unc. bacterium AJ576327	P. ephippiata gut (Coleoptera: Scarabaeidae)
24	Bacteroidetes	16B, (JQ308148)	(92.5) Unc. bacterium FJ674429	Cattle feedlot
			(91.9) Unc. Bacteroidetes AB522123	R. santonensis gut (Isoptera: Termitidae)
			(89.2) Unc. bacterium EF176896	Tipula abdominalis gut (Diptera: Tipulidae)
25	Bacteroidetes	35C, (JQ308149)	(96.2) Unc. bacterium AJ576327	P. ephippiata gut (Coleoptera: Scarabaeidae)
26	Bacteroidetes	64A (JX463097)	(94.2) Unc. bacterium HQ728219	Anode of a glucose-fed microbial fuel cell
26	Bacteroidetes	64A (JX463097)	(93.7) Unc. bacterium AJ576361	P. ephippiata gut (Coleoptera: Scarabaeidae)
27	Bacteroidetes	31C, (JQ308150)	(93.1) Unc. bacterium DQ447343	Urban biowaste
		31C, (JQ308150)	(89.3) Elizabethkingia sp. GU45829	R. speratus gut (Isoptera: Termitidae)
		40C, (JQ308151)	(92.8) Unc. bacterium DQ447343	Urban biowaste
		40C, (JQ308151)	(89.2) Unc. Bacteroidetes HM215036	Bumble bee gut (Hymenoptera: Apidae)
28	Bacteroidetes	17B; 37C; 34C, 59B (JQ308154, JQ308155, JQ308153, JX463099)	(94.9) Unc. Bacteroidetes DQ837639	Apis mellifera gut (Hymenoptera: Apidae)
		55B (JX463095)	(94.6) Unc. Bacteroidetes DQ837639	Apis mellifera gut (Hymenoptera: Apidae)
		56B (JX463094)	(94.8) Unc. Bacteroidetes DQ837639	Apis mellifera gut (Hymenoptera: Apidae)
29	Bacteroidetes	38C, (JQ308152)	(94.3) Unc. Bacteroidetes DQ837639	Apis mellifera gut (Hymenoptera: Apidae)

¹Description of GenBank subjects displaying the top-scoring BLAST alignment results of sequence similarity.
²Animal host or other environment in which the subject having homology with the present sequence is described in GenBank records.
³Unc. = ‘Uncultured’.
OTUs are defined at 97% similarity threshold. Clones ID are followed by letters A,B or C to identify the three insect guts specimens.

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ISSN: 1471-2180

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