Figure 3
BacLight staining of dissected Cansiliella servadeii midgut resuspended material. Live bacterial cells stain in green while insect epithelial nuclei stain in red. In a) clumps of bacteria are seen flowing out from the rupture of the bent gut tract. In b) a different portion is shown and the abundant masses of extracted bacteria. In c) individual bacterial cells are released from the gut epithelium through a hole pierced with forceps. In d) a region of the gut from which several distinct bacterial cells can be seen along with others in more clustered formations. Scale bars: a),b): 350 Ī¼m,c),d): 50 Ī¼m.