Skip to main content

Table 1 Genotypic and phenotypic characterization of S. aureus clinical isolates

From: Erratum to: Exploring the contribution of efflux on the resistance to fluoroquinolones in clinical isolates of Staphylococcus aureus

   QRDR mutationsb MIC (mg/L)c
     EtBr CIP NOR NAL
Isolatea PFGE pattern GrlA GyrA No + + No + + No + + No + +
EI TZ CPZ EI TZ CPZ EI TZ CPZ EI TZ CPZ
ATCC25923 - WT WT 6.25 0.75 0.75 0.25 0.125 0.125 0.5 0.125 0.125 64 n.d. n.d.
ATCC25923EtBr - WT WT 200 25 12.5 1 0.25 0.25 2 0.25 0.25 64 n.d. n.d.
SM1 A2 S80Y/E84G S84L 16 4 4 128 32 64 512 128 256 256 64 64
SM10 A4 S80Y/E84G S84L 16 2 4 128 64 64 512 128 128 128 64 64
SM14 A3 S80Y/E84G S84L 16 4 4 256 32 128 1024 128 256 256 64 64
SM17 A4 S80Y/E84G S84L 16 4 4 256 64 64 1024 256 512 256 64 64
SM25 A1 S80Y/E84G S84L 8 2 4 128 32 64 512 64 128 256 32 64
SM27 A4 S80Y/E84G S84L 16 4 4 256 32 64 512 128 256 256 64 64
SM43 A1 S80Y/E84G S84L 16 2 4 128 64 64 512 128 128 512 256 64
SM46 A1 S80Y/E84G S84L 16 4 4 128 64 64 512 128 256 128 64 64
SM47 A1 S80Y/E84G S84L 8 2 4 256 32 64 512 128 256 256 64 64
SM48 A1 S80Y/E84G S84L 8 4 4 256 32 64 512 128 256 256 64 64
SM50 B1 S80F/E84K S84L 8 1 2 64 16 16 256 32 64 128 64 64
SM52 C1 S80Y S84L 16 1 2 16 8 8 64 32 32 128 32 64
SM2 B2 S80F/E84K S84L 8 2 2 32 16 16 128 32 32 64 16 64
SM3 E1 S80F/E84G S84L 1 1 1 16 8 8 64 32 32 64 16 16
SM4 E2 S80F S84L 4 2 1 8 8 8 64 32 32 64 32 64
SM5 E3 S80F/E84G S84L 4 2 1 32 16 16 128 64 64 64 32 32
SM6 A5 S80F E88K 4 2 1 16 16 16 64 32 32 64 32 32
SM7 E1 S80F S84L 2 2 1 8 8 4 64 32 32 128 32 64
SM8 A5 S80F E88K 4 2 1 16 8 16 128 64 64 128 32 64
SM12 E1 S80F S84L 2 2 1 16 8 8 64 32 32 128 32 64
SM16 A6 S80F E88K 4 2 1 16 16 16 128 32 64 64 32 64
SM22 A1 S80Y/E84G S84L 8 4 4 128 16 32 512 128 128 64 32 64
SM34 D1 S80F/E84K S84L 4 2 2 64 16 32 64 16 32 32 16 32
SM36 E1 S80F S84L 4 2 2 16 8 8 64 16 32 128 32 64
SM40 E1 S80F S84L 8 4 4 32 32 32 512 128 128 16 8 16
  1. aIsolates in bold correspond to the EtBrCW-positive isolates. bWT: wild-type; S: serine; F: phenylalanine; E: glutamate; K: lysine; Y: tyrosine; L: leucine; G: glycine. cValues in bold-type correspond to a MIC decrease of ≥ four-fold in the presence of the efflux inhibitor (EI) in comparison to the values with no EI [10]. The concentration of each EI used is defined in the Methods section. EtBr: ethidium bromide; CIP: ciprofloxacin; NOR: norfloxacin; NAL: nalidixic acid; TZ: thioridazine; CPZ: chlorpromazine; n.d.: not determined.